Rat IgG1 isotype control-InVivo monoclonal antibody (Clone: HRPN) reacts with horseradish peroxidase (HRP). Because HRP is not expressed by mammals this antibody is ideal for use as an isotype-matched control for rat IgG1 antibodies in most in vivo and in vitro applications. This rat IgG1 isotype control has been used by flow cytometric analysis of mouse splenocyte suspension, this can be used at the same concentration as the experimental antibody.
规格
产品名称
Rat IgG1-kappa (Pacific Blue)
别名
大鼠IgG1抗体 | 大鼠IgG1 | 大鼠IgG1同型对照抗体
英文别名
Rat IgG1 | Rat IgG1 Isotype Control | Rat IgG1 antibody | Rat IgG1 Isotype Control antibody
规格或纯度
ExactAb™, 已验证, 重组, 0.5 mg/mL
宿主种属
大鼠(Rat)
偶联
Pacific Blue
Excitation(nm)
Violet Laser(405nm)
Emission(nm)
455nm
Ex/Em(nm)
Ex:410nm;Em:455nm
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein G purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
Rat IgG1-kappa (Pacific Blue) (Ab225936) - Flow Cytometry C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were stained with Rat IgG1-kappa (Pacific Blue) (Ab225936) (right panel). Left panel - Unlabelled control, cells without incubation with primary antibody.
Rat IgG1-kappa (Pacific Blue) (Ab225936) - Flow Cytometry C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with Rat IgG1-kappa (Pacific Blue) (Ab225936) (blue). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
Use at an assay dependent concentration. Each lot of this antibody is quality control tested by flow cytometric analysis as negative control. Use at concentrations comparable to those of the speci