Fluorescent secondary antibodies recognize IgG heavy chains and all classes of immunoglobulin light chains from mouse. Ready-to-use secondary antibody concentrations have been optimized and tested by immunofluorescent staining with Flow Cytometry and IF/ICC. No calculations and dilutions are required. Just add an assay dependent volume to stain your cells, and you're ready for the next step in your experiment.
规格
产品名称
Goat Anti-Mouse IgG H&L (AF532) (Ready to use)
规格或纯度
ExactAb™,即用型,无叠氮钠,已验证,预吸附,见 COA
宿主种属
山羊(Goat)
特异性
Mouse IgG
种属反应性
小鼠(Mouse)
交叉反应
No cross reactivity with Rabbit IgG and Human IgG.
免疫原
Mouse IgG
偶联
AF532
Excitation(nm)
Green Laser(532nm)
Emission(nm)
555nm
Ex/Em(nm)
Ex:530nm;Em:555nm
克隆类型
多克隆抗体
Format
Whole IgG
纯化方法
Antigen affinity purified
浓度
见 COA
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.1% Proclin300.
IF analysis of EpCAM (green) in HT-29 cells. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Catumaxomab (anti-CD3&EpCAM) (Ab175489) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (AF532) (Ready to use) (Ab226611) at 2mL/well for 1 hour at room temperature in the dark (green). A 6-well cell culture plate was used for this experiment. PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with Hoechst 33342 (B113845) (blue) at 1/1000 dilution for 5 minutes. Images were taken on the confocal laser scanning microscope.
Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with Recombinant CD45 Antibody (Ab210608). Goat Anti-Mouse IgG H&L (AF532) (Ready to use) (Ab226611) at 100 μL/well was used as the secondary antibody. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
ZJ26F0434044
危险品化学品经营许可证（带存储）