Fluorescent secondary antibodies recognize IgG heavy chains and all classes of immunoglobulin light chains from rabbit. Ready-to-use secondary antibody concentrations have been optimized and tested by immunofluorescent staining with Flow Cytometry and IF/ICC. No calculations and dilutions are required. Just add an assay dependent volume to stain your cells, and you're ready for the next step in your experiment.
No cross reaction was detected with human IgG, mouse IgG, horse IGG, pig IgG and chicken IGY.
免疫原
Rabbit IgG
偶联
AF405
Excitation(nm)
Violet Laser(405nm)
Emission(nm)
422nm
Ex/Em(nm)
Ex:401nm;Em:422nm
克隆类型
多克隆抗体
Format
Whole IgG
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.1% Proclin300.
防腐剂
0.1% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
This product can be stored at 2-8°C for 12 months. Do not freeze. Store in the dark.
图片
Goat Anti-Rabbit IgG H&L (AF405) (Ready to use) (Ab226635) - IF/ICC IF analysis of HA tag (blue) in Hela cells transfected with an eGFP-HA tag. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant HA tag Antibody (Ab106771) at 0.5 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Rabbit IgG H&L (AF405) (Ready to use) (Ab226635) at 1mL/well for 1 hour at room temperature in the dark (blue). A 24-well cell culture plate was used for this experiment. PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with PI (red). Images were taken on the confocal laser scanning microscope.
Goat Anti-Rabbit IgG H&L (AF405) (Ready to use) (Ab226635) - Flow Cytometry Flow Cytometry analysis of HA tag expression by HEK293T cells transfected with an eGFP-HA tag (red) compared to an isotype control (blue). The cells were fixed with 100% methanol for 5 minutes and then stained with Recombinant HA tag Antibody (Ab106771) at 0.5 μg/mL for 30 minutes at 4°C in the dark. Then incubated with Goat Anti-Rabbit IgG H&L (AF405) (Ready to use) (Ab226635) at 100 μL/well was used as the secondary antibody. Blue - Rabbit IgG (Ab170215).
ZJ25F1028525
危险品化学品经营许可证（带存储）