Mouse Anti-Human IgG Antibody (mAb), Secondary antibody, could be used for IF/ICC and Flow Cytometry.
规格
产品名称
Mouse Anti-Human IgG (AF405)
别名
小鼠抗人IgG抗体 | 小鼠抗人IgG | 鼠抗人IgG抗体 | 鼠抗人IgG
英文别名
Mouse Anti-Human IgG Antibody | Mouse Anti Human IgG Antibody | Anti-Human IgG Antibody | Anti Human IgG Antibody | Human IgG Antibody | FLJ39988 antibodyFLJ40036 antibody | FLJ40253 antibody | FLJ40587 antibody | FLJ40789 antibody | FLJ40834 antibody | G
规格或纯度
ExactAb™, 已验证, 0.5mg/mL
宿主种属
小鼠(Mouse)
特异性
Human IgG
种属反应性
人(Human)
交叉反应
No cross reactivity with mouse IgG, rabbit IgG and goat IgG.
免疫原
Human IgG
偶联
AF405
Excitation(nm)
Violet Laser(405nm)
Emission(nm)
422nm
Ex/Em(nm)
Ex:401nm;Em:422nm
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Octylic acid-ammonium sulfate precipitation
浓度
0.5mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
Mouse Anti-Human IgG (AF405) (Ab226737) - IF/ICC IF analysis of EpCAM (blue) in HT-29 cells. The cells were fixed and permeabilized with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Citatuzumab (anti-EpCAM) (Ab209902) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Mouse Anti-Human IgG (AF405) (Ab226737) at a dilution of 1/500 for 1 hour at room temperature in the dark (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with PI (red). Images were taken on the confocal laser scanning microscope.
Mouse Anti-Human IgG (AF405) (Ab226737) - Flow Cytometry Flow Cytometry analysis of HT-29 cells labelling EpCAM (red) with Citatuzumab (anti-EpCAM) (Ab209902). Mouse Anti-Human IgG (AF405) (Ab226737) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.
ZJ25F1028241
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