Recombinant DYKDDDDK Antibody, clone P16A17, could be used for WB, Flow Cytometry, IF/ICC and so on.
规格
产品名称
Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Biotin)
别名
Flag标签抗体 | DYKDDDDK表位标签 | DYKDDDDK抗体
英文别名
DDDDK epitope tag antibody | DDDK antibody | ddk antibody | DYKDDDDK (also known as the FLAG® tag sequence) antibody | DYKDDDDK antibody | DYKDDDDK epitope tag antibody | DYKDDDDK tag antibody | ECS epitope tag antibody | ECS tag antibody | Enterokinase C
规格或纯度
ExactAb™, 已验证, 重组, 高性能, 0.5 mg/mL
生化机理
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended.
宿主种属
小鼠(Mouse)
特异性
DYKDDDDK epitope tag
种属反应性
不依赖种属(Species independent)
免疫原
Synthetic DYKDDDDK peptide
阳性对照
WB: DYKDDDDK tag transfected HEK293, whole cell lysate Flow Cytometry: HEK293 cells transfected with Flag and mCherry tags IF/ICC: HEK293 cells transfected with Flag and eGFP tags
偶联
Biotin
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
避光,-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
图片
Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Biotin) (Ab229372) - IF/ICC IF analysis of DYKDDDDK tag (Binds to Flag tag) (Red) in HEK293T cells transfected with a EGFP-DYKDDDDK. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Biotin) (Ab229372) at 1/1000 dilution in blocking buffer for 2 hour at RT and then incubated with Recombinant Streptavidin Protein (APC) (rp169048) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Biotin) (Ab229372) - Western Blot All lanes: Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Biotin) (Ab229372) at 1/10000 dilution Samples: Lysates at 20 µg per lane Secondary: SA-HRP (np156148) at 1/10000 dilution Predicted band size: 65, 116 kDa Observed band size: 65, 116 kDa Exposure time: 100.0 s
Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Biotin) (Ab229372) - Flow Cytometry Flow Cytometry analysis of DYKDDDDK tag (Binds to Flag tag) expression by HEK293T cells transfected with a mCherry-DYKDDDDK tag (red) compared to an isotype control (blue). The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 15 minutes and then stained with Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Biotin) (Ab229372) at a dilution of 1/150 for 30 minutes at 4°C in the dark. Then incubated with Recombinant Streptavidin Protein (AF488) (rp181943). Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
ZJ25F0927279
危险品化学品经营许可证（带存储）