Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
宿主种属
小鼠(Mouse)
特异性
Human CD141
种属反应性
人(Human)
阳性对照
Flow Cytometry: Human peripheral blood monocytes
偶联
APC
Excitation(nm)
Red Laser(633nm)
Emission(nm)
660nm
Ex/Em(nm)
Ex:650nm;Em:660nm
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
5 μL/test
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Flow cytometry analysis of Thrombomodulin/CD141 expression by human peripheral blood cells (right panel) compared to an isotype control (left panel). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant Thrombomodulin/CD141 Antibody (APC) (Ab231404) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (APC) (Ab180309).
Flow cytometry analysis of Thrombomodulin/CD141 expression by human peripheral blood monocytes (red) compared to an isotype control (blue). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant Thrombomodulin/CD141 Antibody (APC) (Ab231404) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (APC) (Ab180309). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
5μl/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.