Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance.
Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis.
Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL.
Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation (By similarity).
Modulates B-cell biology through ERK1/2 activation in a Ca2+-dependent pathway via the non-selective Ca2+ channel TRPC1, leading to IL-10 production (By similarity).
宿主种属
大鼠(Rat)
特异性
Mouse CD5
种属反应性
小鼠(Mouse)
免疫原
Mouse Thymus / Spleen
阳性对照
Flow Cytometry: C57BL/6 mouse splenocytes
偶联
PerCP
Excitation(nm)
Blue Laser(488nm)
Emission(nm)
675nm
Ex/Em(nm)
Ex:482nm;Em:675nm
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Flow cytometry analysis of CD5 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were preincubated with Mouse FcR Blocking Reagent (Ab219990). The cells were then stained with Recombinant CD5 Antibody (PerCP) (Ab231926) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (PerCP) (Ab190074).
Flow cytometry analysis of CD5 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were preincubated with Mouse FcR Blocking Reagent (Ab219990). The cells were then stained with Recombinant CD5 Antibody (PerCP) (Ab231926) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (PerCP) (Ab190074). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤1.0μg/test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
ZJ26F0231904
危险品化学品经营许可证（带存储）