May have a pivotal role in cell differentiation of different cell types. May have a specific role in early thymocyte development. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Modulates B-cell activation responses (By similarity).
In association with SIGLEC10 may be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70 and HSP90.
Plays a role in the control of autoimmunity.
宿主种属
大鼠(Rat)
特异性
Mouse CD24
种属反应性
小鼠(Mouse)
免疫原
C57BL/10 Mouse Splenic T Lymphocytes
阳性对照
Flow Cytometry: C57BL/6 mouse splenocytes
偶联
AF405
Excitation(nm)
Violet Laser(405nm)
Emission(nm)
422nm
Ex/Em(nm)
Ex:401nm;Em:422nm
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Flow cytometry analysis of CD24 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were preincubated with Mouse FcR Blocking Reagent (Ab219990). The cells were then stained with Recombinant CD24 Antibody (AF405) (Ab231949) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF405) (Ab190070).
Flow cytometry analysis of CD24 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were preincubated with Mouse FcR Blocking Reagent (Ab219990). The cells were then stained with Recombinant CD24 Antibody (AF405) (Ab231949) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (AF405) (Ab190070). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
ZJ25F1230152
危险品化学品经营许可证（带存储）