PHA-stimulated (3 days) human peripheral blood lymphocytes were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained Recombinant CD103 Antibody (Biotin) (Ab231975) (red) compared to an isotype control (blue). Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody. Unstimulated PBMCs were used as control (left panel).
应用
应用名称
稀释比例
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
ZJ26F0737676
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