Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions.
Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes.
Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions.
Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils.
Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal.
Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes.
Modulates bradykinin receptor BDKRB2 activation.
Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells.
Induces susceptibility to atherosclerosis (By similarity).
Isoform Delta15, Does not protect against apoptosis.
宿主种属
小鼠(Mouse)
特异性
CD31
种属反应性
人(Human)
免疫原
Human CD31
阳性对照
Flow Cytometry: Human peripheral blood lymphocytes
偶联
PE-Cy7
Excitation(nm)
Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)
774nm
Ex/Em(nm)
Ex:565nm;Em:774nm
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
5 μL/test
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Flow cytometry analysis of CD31 expression by human peripheral blood cells (right panel) compared to an isotype control (left panel). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD31 Antibody (PE-Cy7) (Ab231997) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (PE-Cy7) (Ab190079).
Flow cytometry analysis of CD31 expression by human peripheral blood monocytes (red) compared to an isotype control (blue). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD31 Antibody (PE-Cy7) (Ab231997) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (PE-Cy7) (Ab190079). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
5μl/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
ZJ25F1230146
危险品化学品经营许可证（带存储）