Recombinant CD45.2 Antibody, clone P12F26, could be used for Flow Cytometry.
规格
产品名称
Recombinant CD45.2 Antibody (AF405)
别名
白细胞共同抗原 | CD45抗体 | CD45.2单克隆抗体 | Anti-CD45抗体
英文别名
B220 antibody | CD 45 antibody | CD45 antibody | CD45 antigen antibody | CD45R antibody | GP180 antibody | L-CA antibody | LCA antibody | Leukocyte common antigen antibody | loc antibody | Ly-5 antibody | LY5 antibody | Ly5, homolog of antibody | Lyt-4 an
规格或纯度
重组, ExactAb™, 已验证, 0.5 mg/mL
生化机理
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity).
Dephosphorylates LYN, and thereby modulates LYN activity. Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.
宿主种属
小鼠(Mouse)
特异性
Mouse CD45
种属反应性
小鼠(Mouse)
免疫原
B10.S mouse thymocytes and splenocytes
阳性对照
Flow Cytometry: C57BL/6 mouse splenocytes
偶联
AF405
Excitation(nm)
Violet Laser(405nm)
Emission(nm)
422nm
Ex/Em(nm)
Ex:401nm;Em:422nm
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Flow cytometry analysis of CD45.2 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD45.2 Antibody (AF405) (Ab232037) at 0.3μg/test for 1 hour at 4°C. Left panel - Mouse IgG (AF405) (Ab190076).
Flow cytometry analysis of CD45.2 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD45.2 Antibody (AF405) (Ab232037) at 0.3μg/test for 1 hour at 4°C. Blue - Mouse IgG (AF405) (Ab190076). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
ZJ25F1129610
危险品化学品经营许可证（带存储）