Recombinant CD45.2 Antibody, clone P12F26, could be used for Flow Cytometry.
规格
产品名称
Recombinant CD45.2 Antibody (Pacific Blue)
别名
白细胞共同抗原 | CD45抗体 | CD45.2单克隆抗体 | Anti-CD45抗体
英文别名
B220 antibody | CD 45 antibody | CD45 antibody | CD45 antigen antibody | CD45R antibody | GP180 antibody | L-CA antibody | LCA antibody | Leukocyte common antigen antibody | loc antibody | Ly-5 antibody | LY5 antibody | Ly5, homolog of antibody | Lyt-4 an
规格或纯度
重组, ExactAb™, 已验证, 0.5 mg/mL
生化机理
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity).
Dephosphorylates LYN, and thereby modulates LYN activity. Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.
宿主种属
小鼠(Mouse)
特异性
Mouse CD45
种属反应性
小鼠(Mouse)
免疫原
B10.S mouse thymocytes and splenocytes
阳性对照
Flow Cytometry: C57BL/6 mouse splenocytes
偶联
Pacific Blue
Excitation(nm)
Violet Laser(405nm)
Emission(nm)
455nm
Ex/Em(nm)
Ex:410nm;Em:455nm
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Flow cytometry analysis of CD45.2 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were preincubated with Mouse FcR Blocking Reagent (Ab219990). The cells were then stained with Recombinant CD45.2 Antibody (Pacific Blue) (Ab232047) at 0.3μg/test for 1 hour at 4°C. Left panel - Mouse IgG (Pacific Blue) (Ab216764).
Flow cytometry analysis of CD45.2 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were preincubated with Mouse FcR Blocking Reagent (Ab219990). The cells were then stained with Recombinant CD45.2 Antibody (Pacific Blue) (Ab232047) at 0.3μg/test for 1 hour at 4°C. Blue - Mouse IgG (Pacific Blue) (Ab216764). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
ZJ25F1129464
危险品化学品经营许可证（带存储）