ExactAb™,Suitable for Flow Cytometry,无叠氮钠,科研级,重组 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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储存与运输
2-8°C储存,避光,禁止冷冻。低温运输,禁止冷冻 。请查阅批次 COA 获取详细规格。
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质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
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文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
规格
产品名称
Recombinant CD45 Antibody (AF488)
别名
CD45抗体 | 白细胞共同抗原CD45抗体
英文别名
T200 glycoprotein antibody | T200 leukocyte common antigen antibody
规格或纯度
科研级,重组,ExactAb™,无叠氮钠,Suitable for Flow Cytometry,5 μL/test
生化机理
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed:35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
宿主种属
小鼠(Mouse)
特异性
CD45
种属反应性
人(Human)
免疫原
Human PBMC
阳性对照
Flow Cytometry: Human peripheral blood lymphocytes
偶联
AF488
Excitation(nm)
Blue Laser(488nm)
Emission(nm)
525nm
Ex/Em(nm)
Ex:490nm;Em:525nm
作用类型
抗体
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein G purified
浓度
5 μL/test
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Flow cytometry analysis of CD45 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD45 Antibody (AF488) (Ab232096) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF488) (Ab190245).
Flow cytometry analysis of CD45 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD45 Antibody (AF488) (Ab232096) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF488) (Ab190245). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
5μl/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
ZJ26F0332487
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