科研级,重组,ExactAb™,无叠氮钠,Suitable for Flow Cytometry,见COA
生化机理
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed:35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
宿主种属
小鼠(Mouse)
特异性
CD45
种属反应性
人(Human)
免疫原
Human PBMC
阳性对照
Flow Cytometry: Human peripheral blood lymphocytes
偶联
PE-Cy7
Excitation(nm)
Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)
774nm
Ex/Em(nm)
Ex:565nm;Em:774nm
作用类型
抗体
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein G purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Flow cytometry analysis of CD45 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD45 Antibody (PE-Cy7) (Ab232106) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (PE-Cy7) (Ab190079). Black - Unlabelled control, cells without incubation with primary antibody.
Flow cytometry analysis of CD45 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD45 Antibody (PE-Cy7) (Ab232106) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (PE-Cy7) (Ab190079).
应用
应用名称
稀释比例
Flow Cytometry
5 μL/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
ZJ26F0332568
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