ADGRE1 antibody | Adhesion G protein coupled receptor E1 antibody | Adhesion G protein-coupled receptor E1 antibody | AGRE1_HUMAN antibody | Cell surface glycoprotein EMR1 antibody | Cell surface glycoprotein F4/80 antibody | DD7A5 7 antibody | Egf like m
规格或纯度
科研级,重组,ExactAb™,无叠氮钠,Suitable for Flow Cytometry,0.5 mg/mL
生化机理
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
宿主种属
大鼠(Rat)
特异性
F4/80
种属反应性
小鼠(Mouse)
免疫原
Murine macrophages
阳性对照
Flow Cytometry: C57BL/6 Mouse bone marrow cells
偶联
AF405
Excitation(nm)
Violet Laser(405nm)
Emission(nm)
422nm
Ex/Em(nm)
Ex:401nm;Em:422nm
作用类型
抗体
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Two-color flow cytometric analysis of F4/80 expression on C57BL/6 mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Mouse FcR Blocking Reagent (Ab219990). The cells were then stained with Recombinant CD11b Antibody (APC) (Ab231304) and either Rat IgG (AF405) (Ab190070; Left panel) or Recombinant F4/80 Antibody (AF405) (Ab232111; Right panel) at 0.1μg/test.
应用
应用名称
稀释比例
Flow Cytometry
5 μL/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.