Recombinant CD19 Antibody, clone P99G09, could be used for Flow Cytometry.
规格
产品名称
Recombinant CD19 Antibody (Pacific Blue)
别名
白细胞分化抗原19 | CD19抗体
英文别名
Antibody deficiency due to defect in CD19 antibody | Antibody deficiency due to defect in CD19, included antibody | AW495831 antibody | B lymphocyte antigen CD19 antibody | B lymphocyte surface antigen B4 antibody | B-lymphocyte antigen CD19 antibody
规格或纯度
ExactAb™,已验证,重组,0.5 mg/mL
生化机理
Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
宿主种属
大鼠(Rat)
特异性
Mouse CD19
种属反应性
小鼠(Mouse)
免疫原
Mouse CD19-expressing K562 human erythroleukemia cells.
阳性对照
Flow Cytometry: C57BL/6 mouse splenocytes
偶联
Pacific Blue
Excitation(nm)
Violet Laser(405nm)
Emission(nm)
455nm
Ex/Em(nm)
Ex:410nm;Em:455nm
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Flow cytometry analysis of CD19 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). C57BL/6 mouse splenocytes cells were stained with Recombinant CD19 Antibody (Pacific Blue) (Ab302116) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (Pacific Blue) (Ab216768).
Flow cytometry analysis of CD19 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). C57BL/6 mouse splenocytes cells were stained with Recombinant CD19 Antibody (Pacific Blue) (Ab302116) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (Pacific Blue) (Ab216768). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.