Recombinant CD11c Antibody, clone P21C38, could be used for Flow Cytometry.
规格
产品名称
Recombinant CD11c Antibody (PerCP)
规格或纯度
ExactAb™,已验证,重组,5 μL/test
生化机理
Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
宿主种属
小鼠(Mouse)
特异性
CD11c
种属反应性
人(Human)
免疫原
Dendritic cells of synovial fluid
阳性对照
Flow Cytometry: Human peripheral blood monocytes
偶联
PerCP
Excitation(nm)
Blue Laser(488nm)
Emission(nm)
675nm
Ex/Em(nm)
Ex:482nm;Em:675nm
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
5 μL/test
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Flow cytometry analysis of CD11c expression by human peripheral blood cells (right panel) compared to an isotype control (left panel). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD11c Antibody (PerCP) (Ab302139) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (PerCP) (Ab190080).
Flow cytometry analysis of CD11c expression by human peripheral blood monocytes (red) compared to an isotype control (blue). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD11c Antibody (PerCP) (Ab302139) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (PerCP) (Ab190080).
应用
应用名称
稀释比例
Flow Cytometry
5 μL/test. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.