科研级,重组,ExactAb™,无叠氮钠,Suitable for Flow Cytometry,0.5 mg/mL
生化机理
Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
宿主种属
大鼠(Rat)
特异性
Mouse CD8α
种属反应性
小鼠(Mouse)
免疫原
Mouse Spleen Cells or Thymocyte Membranes
阳性对照
Flow Cytometry: C57BL/6 mouse splenocytes
偶联
PerCP
Excitation(nm)
Blue Laser(488nm)
Emission(nm)
675nm
Ex/Em(nm)
Ex:482nm;Em:675nm
作用类型
抗体
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Flow cytometry analysis of CD8α expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were preincubated with Mouse FcR Blocking Reagent (Ab219990). The cells were then stained with Recombinant CD8α Antibody (PerCP) (Ab302187) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (PerCP) (Ab190074).
Flow cytometry analysis of CD8α expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were preincubated with Mouse FcR Blocking Reagent (Ab219990). The cells were then stained with Recombinant CD8α Antibody (PerCP) (Ab302187) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (PerCP) (Ab190074). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤1.0 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
ZJ26F0333552
危险品化学品经营许可证（带存储）