Flow cytometry analysis of CD90.2/Thy1.2 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were preincubated with Mouse Fc Receptor Blocking Solution Plus (M1374079). The cells were then stained with Recombinant CD90.2/Thy1.2 Antibody (AF405) (Ab303122) at 0.03μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF405) (Ab190070).
Flow cytometry analysis of CD90.2/Thy1.2 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were preincubated with Mouse Fc Receptor Blocking Solution Plus (M1374079). The cells were then stained with Recombinant CD90.2/Thy1.2 Antibody (AF405) (Ab303122) at 0.03μg/test for 1 hour at 4°C. Blue - Rat IgG (AF405) (Ab190070). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤1.0 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.