科研级,重组,ExactAb™,无叠氮钠,Suitable for Flow Cytometry,0.5 mg/mL
生化机理
Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as a surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration (By similarity).
宿主种属
大鼠(Rat)
特异性
Mouse CD146
种属反应性
小鼠(Mouse)
免疫原
Endothelial cell line TME-3H3
阳性对照
Flow Cytometry: bEnd.3
偶联
AF405
Excitation(nm)
Violet Laser(405nm)
Emission(nm)
422nm
Ex/Em(nm)
Ex:401nm;Em:422nm
作用类型
抗体
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Flow cytometry analysis of CD146 expression by bEnd.3 cells (red) compared to an isotype control (blue). Cells were stained with Recombinant CD146 Antibody (AF405) (Ab303141) at 0.1μg/test for 1 hour at 4°C. Blue – Rat IgG (AF405) (Ab190070). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤1.0 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
ZJ26F0332556
危险品化学品经营许可证（带存储）