Flow cytometry analysis of Ly-6A/E (Sca-1) expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were preincubated with Mouse Fc Receptor Blocking Solution Plus (M1374079). The cells were then stained with Recombinant Ly-6A/E (Sca-1) Antibody (AF405) (Ab303900) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF405) (Ab190070).
Flow cytometry analysis of Ly-6A/E (Sca-1) expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were preincubated with Mouse Fc Receptor Blocking Solution Plus (M1374079). The cells were then stained with Recombinant Ly-6A/E (Sca-1) Antibody (AF405) (Ab303900) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (AF405) (Ab190070). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
ZJ26F0535561
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