Mouse anti Human/Mouse/Rat ICAM1/CD54 Antibody, Monoclonal (15.2), could be used for Flow Cytometry.
规格
产品名称
ICAM1/CD54 Mouse mAb (PE)
规格或纯度
ExactAb™, 已验证, 5 μL/test
生化机理
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
宿主种属
小鼠(Mouse)
特异性
ICAM1/CD54
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Human infant thymocytes and Sezary cells
阳性对照
Flow Cytometry: human peripheral blood monocytes
偶联
PE
Excitation(nm)
Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)
575nm
Ex/Em(nm)
Ex:565nm;Em:575nm
克隆类型
单克隆抗体
Format
Whole IgG
浓度
5 μL/test
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
ICAM1/CD54 Mouse mAb (PE) (Ab306580) - Flow Cytometry
Flow cytometry analysis of ICAM1/CD54 expression by human peripheral blood cells (right panel) compared to an isotype control (left panel). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with ICAM1/CD54 Mouse mAb (PE) (Ab306580) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (PE) (Ab180308).
ICAM1/CD54 Mouse mAb (PE) (Ab306580) - Flow Cytometry
Flow cytometry analysis of ICAM1/CD54 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with ICAM1/CD54 Mouse mAb (PE) (Ab306580) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (PE) (Ab180308).
应用
应用名称
稀释比例
Flow Cytometry
5 μL/test. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
ZJ26F0434934
危险品化学品经营许可证（带存储）