Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
宿主种属
小鼠(Mouse)
特异性
Transferrin Receptor/CD71
种属反应性
人(Human)
免疫原
Human hematopoietic cell line K562
阳性对照
Flow Cytometry: Jurkat cells
偶联
AF594
Excitation(nm)
Yellow-Green Laser(594nm)
Emission(nm)
618nm
Ex/Em(nm)
Ex:590nm;Em:618nm
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
kappa
浓度
5 μL/test
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
Transferrin Receptor/CD71 Mouse mAb (AF594) (Ab306588) - Flow Cytometry
Flow cytometry analysis of CD71 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with Transferrin Receptor/CD71 Mouse mAb (AF594) (Ab306588) at 5μL/test for 1 hour at 4°C. Blue – Mouse IgG (AF594) (Ab190247). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
5μl/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
ZJ26F0535070
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