Mouse anti Human Transferrin Receptor/CD71 Antibody, Monoclonal (T56/14), could be used for Flow Cytometry.
规格
产品名称
Transferrin Receptor/CD71 Mouse mAb (Biotin)
规格或纯度
ExactAb™,已验证,0.5 mg/mL
生化机理
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
宿主种属
小鼠(Mouse)
特异性
Transferrin Receptor/CD71
种属反应性
人(Human)
免疫原
Human hematopoietic cell line K562
阳性对照
Flow Cytometry: Jurkat cells
偶联
Biotin
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
kappa
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
Transferrin Receptor/CD71 Mouse mAb (Biotin) (Ab306592) - Flow Cytometry
Flow cytometry analysis of CD71 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with Transferrin Receptor/CD71 Mouse mAb (Biotin) (Ab306592) at 5μL/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue – Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
ZJ26F0434642
危险品化学品经营许可证（带存储）