计算溶液所需的质量、体积或浓度。
Lysine-fixable biotin-dextran (MW 10,000) is taken up by neuronal processes and transported bidirectionally. It infiltrates axon boutons in the anterograde direction and dendritic processes in the retrograde direction. Staining can be observed in fixed and sectioned tissue from two days to two weeks after biotin-dextran is injected into the brain or spinal cord. It can also be applied to cut nerve tracts. Biotinylated neurons are detected by either light or electron microscopy following incubation with avidin-horseradish peroxidase conjμgate and the electron-dense substrate 3,3′-diaminobenzidine (DAB).
Lysine-fixable biotin-dextran (MW 10,000) is taken up by neuronal processes and transported bidirectionally. It infiltrates axon boutons in the anterograde direction and dendritic processes in the retrograde direction. Staining can be observed in fixed and sectioned tissue from two days to two weeks after biotin-dextran is injected into the brain or spinal cord. It can also be applied to cut nerve tracts. Biotinylated neurons are detected by either light or electron microscopy following incubation with avidin-horseradish peroxidase conjugate and the electron-dense substrate 3,3′-diaminobenzidine (DAB).