计算溶液所需的质量、体积或浓度。
BioReagent,无菌过滤 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
生物素化抗体稀释液用于稀释检测抗体。在Luminex/Bio-Plex(基于微珠的流式细胞术)实验中,常规操作流程如下:
1. 待测分析物(如细胞因子)被特异性编码微珠捕获。
2. 加入检测抗体,该抗体与分析物的另一表位结合,形成"三明治"复合物。
3. 最后通过链霉亲和素-藻红蛋白实现信号放大与检测。
检测抗体稀释液正是在步骤2中使用,其作用是将浓缩的(通常为生物素标记的)检测抗体稀释至最佳工作浓度。
The Biotin Antibody Diluentis used for dilution of detection antibodies. In Luminex /Bio-Plex (bead-based flow cytometry) experiments, the general procedure is as follows:
1. The analyte (such as a cytokine) is captured on specifically coded micro spheres.
2. A detection antibody is added. This antibody binds to another epitope of the analyte, forming a "sandwich" complex.
3. Finally, signal amplification and detection are achieved using Streptavidin-Phycoerythrin (Streptavidin-PE).
The Detection Antibody Diluent is used precisely in step 2. It is responsible for diluting the concentrated, typically biotin-labeled detection antibody to its optimal working concentration.