计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
血脑屏障通透性测定试剂盒核心是用分光光度法检测物质穿透模拟血脑屏障的浓度，操作简单、成本低，适合快速筛查。无需荧光标记或放射性探针，依赖物质本身的光吸收特性（或配套显色试剂），操作门槛低、仪器普及（实验室常规分光光度计即可）。
| 项目号 | Components | 50T | Storage |
| B1506000A | 标准品A | 2 mL | RT. Store in the dark. |
| B1506000B | 标准品B | 2 mL | RT. Store in the dark. |
| B1506000C | 标准品C | 2 mL | RT. Store in the dark. |
| B1506000D | 标准品D | 2 mL | RT. Store in the dark. |
| B1506000E | 标准品E | 2 mL | RT. Store in the dark. |
| B1506000F | 染色液 | 10 mL | RT |
| B1506000G | 分离液 | 50 mL | RT |
| B1506000H | PBS | 1 L | RT |
自备耗材及试剂：
注射器、组织匀浆器
操作步骤(仅供参考)：
a. 取处理后的实验动物(以小鼠为例)，静脉注射编号为F（1-200μl）的染色液数秒至 1 分钟内，小鼠眼睛、皮肤出现蓝色。0.5～1h 后处死小鼠，取目的脑组织。
b. 脑组织置于 1.5ml 离心管中，加入 1ml PBS，迅速用组织匀浆器将脑组织制成匀浆，4℃离心1000g 离心 10min。
c. 取上清，加入等量编号 G 分离液，混匀，4℃孵育 18～24h。
d. 4℃离心， 2000g 离心 15min。
e. 取适量上述溶液稀释10倍，取1ml稀释后溶液用分光光度计或酶标仪 620 nm 处吸光值(OD 值)。同时测定标准品的A-E的 OD 值，绘制标准曲线。根据标准曲线计算出待测待测样品的染色液含量。
公司分光光度计测定值：
| B1506000 | 标准品A | 标准品B | 标准品C | 标准品D | 标准品E |
浓度 | 39.062mM | 9.766mM | 2.441mM | 0.610mM | 0.152mM |
OD值 | 1.036 | 0.631 | 0.234 | 0.116 | 0.087 |
（只能看相对通透性改变，不能绝对定量）
注意事项：
1、血脑屏障通透性实验中，染色液注射量应根据不同动物以及动物的重量调整。
2、采用低温冷冻离心机进行离心。
3、为了您的安全和健康，请穿实验服并戴一次性手套操作。
The core of the Blood-Brain Barrier (BBB) Permeability Assay Kit lies in using spectrophotometry to detect the concentration of substances that have penetrated the simulated blood-brain barrier. It features simple operation and low cost, making it suitable for rapid screening. No fluorescent labeling or radioactive probes are required; instead, it relies on the inherent light absorption properties of the substances themselves (or supporting chromogenic reagents). It has a low operational threshold and uses widely available equipment (a standard laboratory spectrophotometer is sufficient).
| B1506000 | Components | 50T | Storage |
| B1506000A | Standard Substance A | 2 mL | RT. Store in the dark. |
| B1506000B | Standard Substance B | 2 mL | RT. Store in the dark. |
| B1506000C | Standard Substance C | 2 mL | RT. Store in the dark. |
| B1506000D | Standard Substance D | 2 mL | RT. Store in the dark. |
| B1506000E | Standard Substance E | 2 mL | RT. Store in the dark. |
| B1506000F | Staining Solution | 10 mL | RT |
| B1506000G | Isolation Solution | 50 mL | RT |
| B1506000H | PBS | 1 L | RT |
Self-Prepared Consumables and Reagents:
Syringe
Tissue Homogenizer
Operating Steps (for reference only):
a. Take the treated experimental animals (using mice as an example). Intravenously inject 1–200 μl of staining solution (No. F). Within a few seconds to 1 minute, the eyes and skin of the mice will turn blue. Sacrifice the mice after 0.5–1 hour and collect the target brain tissue.
b. Place the brain tissue in a 1.5 ml centrifuge tube, add 1 ml of PBS, quickly homogenize the brain tissue using a tissue homogenizer, and centrifuge at 1000×g for 10 minutes at 4°C.
c. Take the supernatant, add an equal volume of separation solution (No. G), mix well, and incubate at 4°C for 18–24 hours.
d. Centrifuge at 2000×g for 15 minutes at 4°C.
e. Take an appropriate amount of the above solution and dilute it 10-fold. Take 1 ml of the diluted solution and measure the absorbance (OD value) at 620 nm using a spectrophotometer or microplate reader. Meanwhile, determine the OD values of standards A–E, plot a standard curve, and calculate the content of the staining solution in the test samples based on the standard curve.
Measured Values by the Company's Spectrophotometer:
| B1506000 | Standard Substance A | Standard Substance B | Standard Substance C | Standard Substance D | Standard Substance E |
Concentration | 39.062mM | 9.766mM | 2.441mM | 0.610mM | 0.152mM |
OD Value | 1.036 | 0.631 | 0.234 | 0.116 | 0.087 |
(Only relative changes in permeability can be observed, absolute quantification is not possible)
Precautions:
1. In the blood-brain barrier permeability experiment, the injection volume of the staining solution should be adjusted according to the type of animal and the animal's weight.
2. A low-temperature refrigerated centrifuge should be used for centrifugation.
3. For your safety and health, please wear a lab coat and disposable gloves during operation.
规格
稳定性与储存
Store at RT long term (12 months). Store in the dark.
英文名称
Blood-Brain Barrier Permeability Assay Kit
储存条件
避光,室温
运输条件
常规运输
产品线
生物产品
名称和识别符
分子类型
试剂盒
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0332882 |
分析证书 | B1506000 |
技术文档和文章
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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