丁酰胆碱酯酶(BChE)是一种丝氨酸水解酶，结构上类似于乙酰胆碱酯酶(AChE)，但具有不同的底物特异性和抑制剂敏感性。该酶是由4个相同亚基（每个110kDa）组成的四聚体糖蛋白，又称为拟胆碱酯酶(pseudocholinesterase)。它在肝脏中合成，分布于血液、神经肌肉接头突触、神经胶质细胞和白质轴突中。

应用

马血清中的丁酰胆碱酯酶已用于对氧磷抑制丁酰胆碱酯酶的微量热法研究。这种酶也用于研究胆碱能酶的合成和抑制。选择性抑制 BChE 活性可用于有机磷酸酯的检测。由于人体内 BChE 的血液级别与对毒性神经毒剂的保护程度之间存在相关性，因此其在治疗有机磷毒性方面的应用已显示出临床潜力。胆碱酯酶在阿尔茨海默病中的作用也受到关注。对选择性抑制剂的研究可能为 BChE 在健康和患病个体中角色生理作用提供更清晰的认识。本品已用于某些蔬菜水果中胆脂酶抑制剂的筛选，用于恢复认知功能和改善记忆。它也被用来开发一种丁酰胆碱酯酶和胆碱氧化酶固定化生物嗅探器检测尼古丁。尼古丁抑制 BChE 活性。BChE 活性副产物的减少反映了尼古丁的体积。

生化 / 生理作用

丁酰胆碱酯酶(BChE)基因突变会导致呼吸衰竭、长期瘫痪和呼吸暂停。

不同于乙酰胆碱酯酶，丁酰胆碱酯酶(BChE)可高效水解较大的胆碱酯，如丁酰胆碱和苯甲酰胆碱。酶活性可被Ca²⁺和Mg²⁺激活，在pH 6.0-8.0范围内保持恒定，受到甜菜碱、尼古丁、有机磷和氨基甲酸酯抑制。

物理形态

含缓冲盐的冻干粉

制备说明

本品采用硫酸铵分级法从马血清中制得，以冻干粉形式提供。

Butyrylcholinesterase (BChE) is a serine hydrolase that is structurally similar to acetylcholinesterase (AChE), but differs in substrate specificities and inhibitor sensitivities. The enzyme is a tetrameric glycoprotein with four equal subunits (110 kDa each). It is also termed as pseudocholinesterase. It is produced in the liver. It is found in blood, the synapse of neuromuscular junctions and glia cells and axons of white matter.

Application

Butyrylcholinesterase from equine serum has been used in a microcalorimetric study of the inhibition of butyrylcholinesterase by paraoxon. This enzyme has also been used in a study to investigate the synthesis and inhibition of cholinergic enzymes. Selective inhibition of BChE activity can be used in the detection of organophosphates. Its use in the treatment of organophosphate toxicity has shown clinical potential, as there is a correlation between the blood level of BChE in humans and the degree of protection against toxic nerve agents. There has also been an interest in the roles of cholinesterases with regard to Alzheimer′s disease. Investigations into selective inhibitors may provide a clearer picture of the physiological role of BChE in both healthy and diseased individuals. This product has been used for the screening of cholineesterase inhibitors in selected fruits and vegetables, for restoring cognitive function and improving memory. It has also been used to develop a butyrylcholinesterase and choline oxidase immobilized bio-sniffer for the detection of nicotine. Nicotine inhibits BChE activity. A decrease in the byproducts of BChE activity reflects the volume of nicotine.

Biochem/physiol Actions

Butyrylcholinesterase (BChE) can, unlike AChE, efficiently hydrolyze larger esters of choline such as butyrylcholine and benzoylcholine. The enzyme is activated by Ca²⁺ and Mg²⁺ and the activity is constant over the pH range 6.0-8.0. It is inhibited by betaine, nicotine, organophosphates, and carbamates.

Mutations in butyrylcholinesterase (BChE) gene results in the failure of respiratory system, prolonged paralysis and apnea.

Physical form

Lyophilized powder containing buffer salts

Preparation Note

This product is prepared from equine serum using ammonium sulfate fractionation and supplied as a lyophilized powder.

规格

产品名称

丁酰胆碱酯酶 (BChE)

别名

拟胆碱酯酶 | 胆碱脂酶 | 丁酰基, 酰胆碱酰基水解酶

英文别名

规格或纯度

生物活性,ActiBioPure™,天然,高性能,EnzymoPure™,来源于马血清; 蛋白含量: 见COA; ≥10 U/mg protein

生化机理

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters (By similarity).

生物活性

≥10 U/mg protein

种属

马(Horse)

预测分子量

Tetramer 440 kDa

CAS编号和信息

9001-08-5

酶学委员会编号

EC 3.1.1.8

分子类型

酶

产品线

生物产品

储存与运输

物理形态

冻干(Lyophilized)

浓度

来源于马血清; 蛋白含量: 见COA; ≥10 U/mg protein

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

长期储存-20℃；复溶后建议分装，避免反复冻融。

单位定义

一个单位将在 pH8.0，37°C 下每分钟将1.0μ 摩尔丁酰胆碱水解为胆碱和丁酸盐。使用丁酰胆碱作为底物获得的活性是使用乙酰胆碱获得的活性的约 2.5 倍。

高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

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批号(Lot Number)	证书类型	货号
ZJ26F0737183	分析证书	B1510978
ZJ26F0434231	分析证书	B1510978
ZJ26F0434230	分析证书	B1510978

技术文档和文章

胆碱酯酶类检测体系的反应原理、方法类型与实验设计

目录：技术文章

探索主题:

DTNB Ellman法荧光法电化学法酶活检测 AChE BChE ChE 胆碱酯酶乙酰胆碱酯酶丁酰胆碱酯酶硫代胆碱底物组织化学法

Cholinesterase Detection Systems: Reaction Principles, Method Types, and Experimental Design

目录：技术文章

探索主题:

DTNB AChE BChE ChE Cholinesterase Acetylcholinesterase Butyrylcholinesterase Ellman method Thiocholine Substrates Enzyme Activity Detection Fluorescence Method Electrochemical Method Histochemical Method

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