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血磷主要指血中的无机磷，以无机磷盐的形式存在。血浆中钙、磷浓度关系密切，在以mg/dL表示时，二者的乘积(［Ca］×［P］)为30～40。当(［Ca］×［P］)>40，则钙和磷以骨盐形式沉积于骨组织；若(［Ca］×［P］)<35则妨碍骨的钙化，甚至可使骨盐溶解，影响成骨作用。血钙和血磷含量的相对稳定依赖于钙、磷的吸收与排泄和钙化及脱钙两种代谢的相对平衡。上述平衡受到维生素D3、甲状旁腺素和降钙素等激素的调节。 测定原理：去除血清中有机磷后，无机磷盐与钼酸铵试剂生成磷钼酸，被硫酸亚铁还原后呈蓝色，在620nm有光吸收；通过测定620 nm吸光度，计算血液中磷含量。
Blood phosphorus mainly refers to inorganic phosphorus in the blood, existing in the form of inorganic phosphate salts. The concentrations of calcium and phosphorus in plasma are closely related; when expressed in mg/dL, their product ([Ca] × [P]) is 30–40. When ([Ca] × [P]) > 40, calcium and phosphorus are deposited in bone tissue as bone salt; if ([Ca] × [P]) < 35, bone calcification is impaired, and bone salt may even dissolve, affecting osteogenesis. The relative stability of blood calcium and blood phosphorus levels depends on the relative balance between the absorption and excretion of calcium and phosphorus, and between calcification and decalcification. These balances are regulated by hormones such as vitamin D₃, parathyroid hormone, and calcitonin. Determination principle: After removing organic phosphorus from serum, inorganic phosphate salts react with ammonium molybdate reagent to form phosphomolybdic acid, which is reduced by ferrous sulfate to produce a blue color with light absorption at 620 nm. The phosphorus content in blood is calculated by measuring the absorbance at 620 nm.
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