BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
谷物淀粉中的直链淀粉最常见的测定方法是对直链淀粉的碘结合能力进行电位法、电流法或比色法测量,从而形成直链淀粉-碘包合复合物。谷物淀粉中的支链淀粉也会与碘形成支链淀粉-碘复合物,它们会降低通过非比色法测量的游离碘的浓度,并且可能会在与比色法中的直链淀粉-碘复合物相似的波长处吸收。 本试剂盒利用ConA只与支链淀粉结合而不与直链淀粉结合的特性,使其分离,再用仅水解淀粉的酶复合物使淀粉水解为葡萄糖,通过检测葡萄糖含量得到直链、支链和总淀粉的含量。
The most common method for determining amylose in cereal starches involves measuring the iodine-binding capacity of amylose by potentiometric, amperometric, or colorimetric methods, thereby forming an amylose-iodine inclusion complex. Amylopectin in cereal starches also forms an amylopectin-iodine complex, which can reduce the concentration of free iodine measured by non-colorimetric methods and may absorb at wavelengths similar to those of the amylose-iodine complex in colorimetric methods. This kit utilizes the property that ConA binds specifically to amylopectin but not to amylose to achieve separation. An enzyme complex that specifically hydrolyzes starch is then used to convert starch into glucose. The amylose, amylopectin, and total starch contents are determined by measuring the glucose concentration.
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