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菠萝蛋白酶(Bromelain)简称菠萝酶，亦称凤梨酶、凤梨酵素等，是存在于菠萝植株中的蛋白水解酶，可从菠萝果茎、叶、皮提取出来，经精制、提纯、浓缩、酶固定化、冷冻干燥而得到，菠萝蛋白酶已被广泛应用于食品、医药等行业，利用吸附力、离子键、共价键等不同的联结方式，将酶与不溶性载体联结制成固定化酶，可以很好的避免酶的失活，易于储存运输。 该试剂盒检测原理是通过菠萝蛋白酶催化底物酪蛋白水解生成酪氨酸，酪氨酸含有酚羟基，可与福林试剂发生福林反应，即福林试剂在碱性条件下容易定量的被酚类化合物还原，生成钨蓝和钼蓝的混合物呈现不同深浅的蓝色，检测680nm处检测吸光度，吸光度的大小与酪氨酸含量的多少有关，吸光度大说明酪氨酸含量高，也就是说菠萝蛋白酶分解的酪蛋白多，酶活性高，主要用于检测植物组织尤其是菠萝匀浆液中菠萝蛋白酶的活性。本产品仅用于科研领域，不适用于临床诊断或其他用途。
Bromelain, often referred to simply as bromelain, is a proteolytic enzyme found in pineapple plants. It can be extracted from the fruit stem, leaves, and peel of pineapples, and is obtained through processes such as purification, concentration, enzyme immobilization, and freeze-drying. Bromelain is widely used in the food, pharmaceutical, and other industries. By using different coupling methods such as adsorption, ionic bonds, and covalent bonds, the enzyme can be linked to an insoluble carrier to prepare immobilized enzymes, which effectively prevents enzyme inactivation and facilitates storage and transport. The detection principle of this kit is based on the hydrolysis of the substrate casein catalyzed by bromelain, producing tyrosine. Tyrosine contains a phenolic hydroxyl group that can react with Folin reagent in the Folin reaction. Specifically, under alkaline conditions, Folin reagent is readily reduced quantitatively by phenolic compounds, producing a mixture of tungsten blue and molybdenum blue, which exhibits varying shades of blue. The absorbance is measured at 680 nm. The magnitude of the absorbance is related to the amount of tyrosine; a higher absorbance indicates a higher tyrosine content, meaning more casein has been broken down by bromelain, reflecting higher enzyme activity. This kit is primarily used for detecting the activity of bromelain in plant tissues, especially in pineapple homogenates. This product is intended for research purposes only and is not suitable for clinical diagnosis or other applications.
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