B5培养基为植物组培常用基础培养基,各类大量元素、微量元素及有机组分配比协调,营养配比均衡,重复性强。本品具有溶解性能优良,体系稳定性佳,不易产生沉淀等多种特点。还可根据试验需求搭配不同植物调节剂,普遍适用于多种作物的组织培养与植株再生体系构建。
使用方法
1、配制溶解:精确称取本品30.21 g,加入1 L蒸馏水或去离子水中,充分搅拌至粉末完全溶解,配制成均匀的培养基溶液。
2、pH调节(可选):若需调节pH值,可在灭菌前使用稀NaOH或者稀HCl溶液进行微调。植物组织培养常用pH范围为5.5~5.8,具体参照实验方案执行。调节时需逐滴添加、边加边搅拌并实时检测pH,避免过度反复调节。
3、灭菌处理:将配制好的培养基分装至合适培养容器,于116 ℃高压蒸汽灭菌30 min。
4、冷却使用:灭菌后置于洁净无菌环境自然冷却至室温,全程保持无菌操作,即可用于植物组织培养实验。
注意事项
1、本品为植物组织培养全无机盐培养基,建议采用室温水溶解后再进行高压灭菌,不建议加热溶解;灭菌后溶液若出现少量沉淀为正常现象,不影响实验使用。
2、产品需置于室温、避光环境密封保存,有效期3年。
3、本产品仅供科研实验使用,严禁用于医药、临床诊断与治疗、食品、化妆品等相关领域,请勿存放于普通住宅区。
4、因用户非正常操作、违规使用本产品所产生的一切意外与损失,本公司不承担相关责任。
B5 medium is a commonly used basal medium for plant tissue culture. It contains well-proportioned macroelements, trace elements and organic components with balanced nutrition and high experimental reproducibility. This product boasts excellent solubility, good system stability and low precipitation tendency. It can be supplemented with different plant growth regulators according to experimental requirements, and is widely suitable for tissue culture and plant regeneration system establishment of various crops.
Instructions for Use
1. Preparation and Dissolution: Accurately weigh 30.21 g of this product, add it to 1 L of distilled or deionized water, and stir thoroughly until the powder dissolves completely to prepare a homogeneous medium solution.
2. pH Adjustment (Optional): If pH adjustment is required, finely tune the pH with dilute NaOH or dilute HCl solution before sterilization. The commonly applicable pH range for plant tissue culture is 5.5~5.8, subject to specific experimental protocols. Add the solution dropwise while stirring and monitor the pH in real time to avoid over-adjustment.
3. Sterilization Treatment: Dispense the prepared medium into appropriate culture vessels, then autoclave at 116 °C under high-pressure steam for 30 minutes.
4. Cooling and Application: After sterilization, let the medium cool naturally to room temperature in a clean sterile environment, mix gently, and maintain aseptic technique throughout all operations. The medium is then ready for plant tissue culture experiments.
Precautions:
1. This product is a full inorganic salt medium for plant tissue culture. It is recommended to dissolve the powder in room-temperature water prior to autoclaving; heating for dissolution is not advised. Minor precipitation after sterilization is normal and will not interfere with experimental results.
2. Store the product hermetically sealed at room temperature away from light. The shelf life is 3 years.
3. This product is for research laboratory use only. It is strictly prohibited for medical purposes, clinical diagnosis and treatment, food, cosmetics and other related fields. Do not store it in residential areas.
4. Our company shall not be liable for any accidents or losses arising from improper operation or unauthorized use of this product by users.