计算溶液所需的质量、体积或浓度。
BioReagent,适用于植物细胞培养 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
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使用说明
1、配制溶解:精确称取本品3.21 g,加入1 L蒸馏水或去离子水中,充分搅拌至粉末完全溶解,配制成均匀的培养基溶液。
2、pH调节(可选):若需调节pH值,可在灭菌前使用稀NaOH或者稀HCl溶液进行微调。植物组织培养常用pH范围为5.5~5.8,具体参照实验方案执行。调节时需逐滴添加、边加边搅拌并实时检测pH,避免过度反复调节。
3、灭菌处理:将配制完成的培养基分装至合适培养容器,于121 ℃高压蒸汽灭菌15 min。
4、冷却使用:灭菌结束后置于洁净无菌环境中自然冷却,全程严格遵守无菌操作规范,即可用于植物组织培养实验。
注意事项
1、本品为植物组织培养全无机盐培养基,建议采用室温水溶解后再进行高压灭菌,不建议加热溶解;灭菌后溶液若出现少量沉淀为正常现象,不影响实验使用。
2、产品需置于室温、避光环境密封保存,有效期3年。
3、本产品仅供科研实验使用,严禁用于医药、临床诊断与治疗、食品、化妆品等相关领域,请勿存放于普通住宅区。
4、因用户非正常操作、违规使用本产品所产生的一切意外与损失,本公司不承担相关责任。
Basal Medium (without agar and sucrose), also known as Gamborg’s B5 Basal Salt Medium, features a classic formula with well-balanced nutrients. Free of agar and sucrose, it allows flexible supplementation of agar, sucrose and various plant growth regulators as required. It is widely applicable to the tissue culture of diverse plant species with great operational flexibility.
Product Components:
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Instructions for Use:
1. Preparation and Dissolution: Accurately weigh 3.21 g of this product and add it to 1 L of distilled or deionized water. Stir thoroughly until the powder is completely dissolved to prepare a homogeneous culture medium solution.
2. pH Adjustment (Optional): If pH adjustment is required, finely tune the pH with dilute NaOH or dilute HCl solution before sterilization. The commonly applicable pH range for plant tissue culture is 5.5~5.8, subject to specific experimental protocols. Add the solution dropwise while stirring and monitor the pH in real time to avoid over-adjustment.
3. Sterilization Treatment: Dispense the prepared culture medium into suitable culture vessels and perform high-pressure steam sterilization at 121 ℃ for 15 minutes.
4. Cooling and Application: After sterilization, place the vessels in a clean and sterile environment for natural cooling. Strictly follow aseptic operation specifications throughout the process. The medium is then ready for plant tissue culture experiments.
Precautions:
1. This product is a full inorganic salt medium for plant tissue culture. It is recommended to dissolve the product at room temperature prior to high-pressure sterilization; heating dissolution is not advised. A small amount of precipitate in the solution after sterilization is a normal phenomenon and will not affect experimental results.
2. The product shall be stored in a sealed container at room temperature, protected from light, with a shelf life of 3 years.
3. This product is for scientific research and experimental use only. It is strictly prohibited for use in medicine, clinical diagnosis and treatment, food, cosmetics and other related fields. Do not store it in residential areas.
4. The manufacturer shall not be liable for any accidents or losses arising from improper operation or unauthorized use of this product by the user.
| pH | 5.5 |
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通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | B1524320 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Suitable for plant cell culture 级别指南 →