计算溶液所需的质量、体积或浓度。
BioReagent,即用型 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
固定的目的在于保存细胞和组织的原有形态结构,固定剂能阻止内源性溶酶体酶对自身组织和细胞的自溶、抑制细菌和霉菌的生长,固定剂通过凝固、生成添加化合物等使蛋白质内部结构发生改变,从而使酶失活。固定液分为醛类固定液、汞类固定液、醇类固定液、氧化剂类固定液、苦味酸盐类固定液等,较为常用的是醛类中的福尔马林、醇类中的乙醇。
BS 固定液主要由甲醇、乙酸等混合而成,固定后对于细胞核染色固定,尤其适用于细胞核 DNA 染色的固定。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤 (仅供参考)
1、按实验具体要求操作。
2、血涂片或细胞涂片置于提前预热至 30~40℃的BS 固定液,固定 20~30min。
3、进行下游染色实验。
注意事项
1、组织取材的厚度不同,固定时间也不同,对组织恰当的选材有利于固定液的渗透。
2、固定液的容量应足够,一般固定液与组织块的体积比率应大于 10:1。
3、取出新鲜组织后,应及时固定,无法及时固定时,应保存于生理盐水中及时送检。
4、为了您的安全和健康,请穿实验服并戴一次性手套操作。
5、试剂开封后请尽快使用,以防影响后续实验效果。
The purpose of fixation is to preserve the original morphological structure of cells and tissues. Fixatives can prevent autolysis of tissues and cells caused by endogenous lysosomal enzymes and inhibit the growth of bacteria and molds. Fixatives alter the internal structure of proteins through coagulation and adduct formation, thereby inactivating enzymes. Fixatives are classified into aldehyde fixatives, mercury‑containing fixatives, alcohol fixatives, oxidant fixatives, picrate fixatives and others. Among them, formalin (aldehyde‑based) and ethanol (alcohol‑based) are widely used.
BS Fixative is mainly composed of methanol, acetic acid and other components. It delivers good fixation performance for cell nuclei, and is especially suitable for fixation for nuclear DNA staining. This reagent is for research use only and is not intended for clinical diagnosis or other applications.
Operating Procedures (For Reference Only)
1. Operate according to specific experimental requirements.
2. Place blood smears or cell smears in pre‑warmed BS Fixative (30‑40 °C) and fix for 20‑30 min.
3. Proceed to downstream staining experiments.
Precautions
1. Fixation time varies with the thickness of tissue samples. Proper tissue sampling facilitates the penetration of fixative.
2. Use a sufficient volume of fixative; generally, the volume ratio of fixative to tissue block should be greater than 10:1.
3. Fresh tissues should be fixed immediately after collection. If immediate fixation is not feasible, store samples in normal saline for prompt processing.
4. For your personal safety and health, wear a lab coat and disposable gloves during operation.
5. Use the reagent as soon as possible after opening to avoid compromising experimental results.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | B1526194 |