计算溶液所需的质量、体积或浓度。
天然 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
缓冲液组分: 20 mM HEPES-HCl, 0.15 M NaCl, 50% glycerol, pH7.4.
百分消光系数(1%):9.6 / 17.4
分子量:330000;94000;74000;71000;31000 Da
蛋白浓度检测方法:BCA 法
Purified from freshly collected bovine plasma using a combination of salt precipitations and column chromatography. This protein is a mixture of Factor V/Va and intermediate activation products as judged by 4–20% gradient gels. Bovine Factor Va functions as a cofactor with the serine protease Factor Xa in the activation of prothrombin. This protein may be used as a source of V/Va in the prothrombinase complex. All bovine raw material is sourced from U.S. cows ≤ 30 months of age.
Buffer Composition: 20 mM HEPES-HCl, 0.15 M NaCl, 50% glycerol, pH7.4.
Extinction Coefficient (1%): 9.6 / 17.4
Molecular Weight: 330,000; 94,000; 74,000; 71,000; 31,000 Da
Protein Concentration determined by BCA assay
The Bovine Factor V/Va is a mixture of Factor V/Va and intermediate activation products as judged by 4–20% gradient gels. Bovine Factor Va functions as a cofactor with the serine protease Factor Xa in the activation of prothrombin. This protein may be used as a source of Factor V/Va in the prothrombinase complex