BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
采用CHOD-PAP终点法结合经典GPO Trinder酶学反应进行测定，胆固醇酯酶（CHE）能够将胆固醇酯分解为游离胆固醇，胆固醇氧化酶（COD）进一步将游离胆固醇氧化为胆甾烯酮和H 2 O 2 ，H 2 O 2 能够在过氧化物酶（POD）的催化作用下与4-氨基安替吡碄（4-AAP）反应生成红色苯醌亚胺，产物在550 nm处具有特征吸收峰，通过吸光值变化即可定量检测低密度脂蛋白胆固醇的含量。
The assay adopts the CHOD-PAP endpoint method combined with the classic GPO-Trinder enzymatic reaction. Cholesteryl esterase (CHE) decomposes cholesteryl esters into free cholesterol, which is further oxidized by cholesterol oxidase (COD) to cholestenone and H₂O₂. Catalyzed by peroxidase (POD), H₂O₂ reacts with 4-aminoantipyrine (4-AAP) to form red quinoneimine. This product has a characteristic absorption peak at 550 nm, and the content of low-density lipoprotein cholesterol can be quantitatively determined by measuring the change in absorbance.
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