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高密度脂蛋白胆固醇（HDL-C）是血清蛋白之一，主要由肝脏合成。它能够运载周围组织中的胆固醇，将其转化为胆汁酸或直接通过胆汁从肠道排出，因此是一种抗动脉粥样硬化的血浆脂蛋白，被誉为“血管清道夫”。HDL-C是冠心病的保护因子，其水平对冠心病的临床诊断具有重要参考价值。 该产品用于检测生物样本中HDL-C含量，检测原理如下： 1、沉淀分离：用沉淀剂分离血清中的高密度脂蛋白胆固醇。 2、水解反应：胆固醇酯酶催化胆固醇酯水解，生成游离胆固醇（FC）和游离脂肪酸（FFA），将胆固醇酯转化为FC。 3、氧化反应：胆固醇氧化酶催化FC氧化，生成Δ⁴-胆甾烯酮和过氧化氢（H₂O₂）。 4、显色反应：过氧化物酶催化H₂O₂氧化4-氨基安替比林和酚，生成红色醌类化合物，该产物在500 nm处具有特征吸收峰。
High‑density lipoprotein cholesterol (HDL‑C) is one of the serum lipoproteins, primarily synthesized by the liver. It is capable of transporting cholesterol from peripheral tissues, converting it into bile acids or excreting it directly via bile through the intestinal tract. Therefore, it is an anti‑atherosclerotic plasma lipoprotein, often referred to as the "vascular scavenger." HDL‑C is a protective factor against coronary heart disease, and its level provides important reference value for the clinical diagnosis of coronary heart disease. This product is used for the determination of HDL‑C content in biological samples. The detection principle is as follows: ① Precipitation separation: A precipitating reagent is used to separate high‑density lipoprotein cholesterol from serum. ② Hydrolysis reaction: Cholesterol esterase catalyzes the hydrolysis of cholesteryl esters to free cholesterol (FC) and free fatty acids (FFA), converting cholesteryl esters into FC. ③ Oxidation reaction: Cholesterol oxidase catalyzes the oxidation of FC, generating Δ⁴‑cholestenone and hydrogen peroxide (H₂O₂). ④ Colorimetric reaction: Peroxidase catalyzes the oxidation of 4‑aminoantipyrine and phenol by H₂O₂, generating a red quinone compound. This product exhibits a characteristic absorption peak at 500 nm.
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