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BioReagent,无菌过滤 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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本产品为 10×BU 缓冲液,pH 为 7.0,经过过滤除菌处理。可用于配制 X-gal 显色培养基,检测 EGY48 酵母单杂交系统中 LacZ 报告基因。
10×BU溶液 (pH=7.0,无菌)为酵母 X-gal 显色实验提供缓冲环境,使酵母菌落显色更清晰、持久。
使用说明 (以500 mL体系为例):
提前在 55 ℃预热20%半乳糖溶液(无菌),棉子糖溶液 (20%, 过滤除菌) (阿拉丁货号:R1524582)以及10×BU溶液 (pH=7.0,无菌)。灭菌后的500 mL培养基降温至55 ℃,依次加入50 mL 20%半乳糖溶液(无菌),25 mL 棉子糖溶液 (20%, 过滤除菌),50 mL 10×BU溶液 (pH=7.0,无菌)以及2 mL X-Gal 溶液 (20 mg/mL) (阿拉丁货号:X1520260),混匀后倒板,凝固后放至2-8℃避光保存以供后续使用。
注意事项:
1. 注意无菌操作,避免微生物污染。
2. 本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。
3. 为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
This product is a 10×BU buffer at pH 7.0 and has been filter-sterilized. It can be used to prepare X-gal chromogenic medium for detecting the LacZ reporter gene in the EGY48 yeast one-hybrid system.
10×BU solution (pH = 7.0, sterile) provides a buffering environment for yeast X-gal chromogenic experiments, making yeast colonies develop clearer and more persistent color.
Instructions for Use (using a 500 mL system as an example):
Preheat a 20% galactose solution (sterile), a raffinose solution (20%, filter-sterilized) (Aladdin Catalog No.: R1524582), and the 10×BU solution (pH = 7.0, sterile) at 55 ℃ in advance. Cool the sterilized 500 mL medium to 55 ℃, then sequentially add 50 mL of 20% galactose solution (sterile), 25 mL of raffinose solution (20%, filter-sterilized), 50 mL of 10×BU solution (pH = 7.0, sterile), and 2 mL of X-Gal solution (20 mg/mL) (Aladdin Catalog No.: X1520260). Mix well and pour plates. After solidification, store at 2–8 ℃ protected from light for subsequent use.
Precautions:
1.Pay attention to aseptic operation to avoid microbial contamination.
2.This product is for research use only. Do not use it for pharmaceutical, clinical diagnostic or therapeutic, food, or cosmetic purposes.
3.For your safety and health, please wear a laboratory coat and disposable gloves and a mask during operation.
| pH | 7 |
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