计算溶液所需的质量、体积或浓度。
致畸试验骨组织保存液
有货
概述
致畸试验骨组织保存液(Bone Tissue Preservation Solution for Teratogenicity Testing)，主要成分为甘油，无色澄清粘稠液体，无腐蚀性。
作用原理：高浓度甘油可隔绝空气、抑制微生物滋生，维持胎仔骨骼与软组织形态稳定，防止标本干瘪、变形、霉变，适配致畸试验骨骼染色透明完成后的标本长期封存、镜检观察。
适用范围：用于食品、化妆品、保健品等毒理学安全性评价，用于大鼠、小鼠、家兔致畸试验胎仔骨骼透明脱色完成后的标本长期浸泡保存。
该试剂仅用于科研领域，不适用于临床诊断或其他用途。
操作步骤(仅供参考)：
1、前置要求：胎仔需完成预处理→透明→茜素红骨骼染色→梯度脱色全部流程。
1)大鼠、小鼠取材规则：取每窝 1/2 胎仔进行骨骼制片；
2)家兔致畸试验不可分半取样，全部胎仔均需制片处理。
2、标本封存保存(本品直接使用，无需稀释)：
1)经过透明液 A(B1529923)、透明液 B(B1529922)脱色完毕的胎仔，完全浸没于本品中，室温避光浸泡 2-3d 充分置换组织水分。
2)浸泡完成后，可长期放置于本品内避光存放，随时取出在体视显微镜下观察骨骼畸形。
3、镜检观察：
1)两级透明完成后，将标本转移至本品中长期封存，便于镜检与标本留存；
2)采用透射光源，体视显微镜下依次检查颅骨、脊柱、肋骨、胸骨、四肢骨，记录骨化缺失、骨骼融合、肋骨分叉、骨缺损、骨化发育不全等各类骨骼畸形；
3)毒理试验统计要求：所有骨骼畸形、发育异常指标均以窝为单位进行统计，计算总畸胎率、单项骨骼畸形率。
注意事项：
1、本品以甘油为基底，低温环境容易结晶凝固，冬季建议室温存放，结晶融化后不影响使用效果。
2、长期保存标本时，容器务必拧紧密封，防止甘油挥发造成标本脱水干瘪。
3、仅用于致畸骨骼标本最终封存，不可用于前期软组织透明、染色工序。
4、若保存液内出现菌丝、浑浊、悬浮物，及时更换全新保存液。
5、配套完整流程：KOH 透明液透明→茜素红染色→透明液 A 脱色→透明液 B 过渡→本品长期保存；另一半胎仔可用致畸试验固定液(内脏检查)(T1529295)做内脏致畸检测。
6、为了您的安全和健康，请穿实验服并戴一次性手套操作。
Bone Tissue Preservation Solution for Teratogenicity Testing is mainly composed of glycerol. It is a colorless, clear, viscous liquid and is non-corrosive.
Principle: High-concentration glycerol can isolate the specimen from air and inhibit microbial growth, maintain the morphological stability of fetal bones and soft tissues, and prevent specimens from shrinking, deforming, or developing mildew. It is suitable for long-term preservation and microscopic observation of specimens after completion of skeletal staining and clearing in teratogenicity testing.
Scope of Application: Used for toxicological safety evaluation of foods, cosmetics, health products, and other products, and for long-term immersion preservation of fetal specimens from rats, mice, and rabbits after skeletal clearing and decolorization in teratogenicity tests.
This reagent is intended for research use only and is not suitable for clinical diagnosis or other purposes.
Operating Procedure (For Reference Only):
1. Prerequisites: Fetuses must complete the entire procedure of pretreatment → clearing → Alizarin Red skeletal staining → graded decolorization.
1) Sampling rule for rats and mice: Select 1/2 of the fetuses from each litter for skeletal preparation;
2) For rabbit teratogenicity tests, half-litter sampling is not applicable, and all fetuses should undergo skeletal preparation.
2. Specimen Preservation (Use Directly Without Dilution):
1) After decolorization with Bone Tissue Clearing Solution A (KOH-Glycerol Solution) for Teratogenicity Testing (B1529923) and Bone Tissue Clearing Solution B (Glycerol Solution) for Teratogenicity Testing (B1529922), completely immerse the fetuses in this product and incubate at room temperature protected from light for 2-3 d to fully replace tissue water;
2) After immersion, specimens may be stored long-term in this product protected from light and removed at any time for observation of skeletal malformations under a stereomicroscope.
3. Microscopic Examination:
1) After completion of the two-step clearing process, transfer the specimens to this product for long-term preservation to facilitate microscopic examination and specimen retention;
2) Using transmitted light, sequentially examine the skull, vertebral column, ribs, sternum, and limb bones under a stereomicroscope, and record various skeletal abnormalities, including ossification defects, skeletal fusion, bifurcated ribs, bone defects, and incomplete ossification;
3) Statistical requirements for toxicological testing: All skeletal malformations and developmental abnormalities should be statistically analyzed using the litter as the unit, and the total fetal malformation rate and individual skeletal malformation rates should be calculated.
Precautions:
1. This product is glycerol-based and may crystallize and solidify at low temperatures. Room-temperature storage is recommended in winter. Crystallization does not affect product performance after melting.
2. For long-term specimen storage, ensure that the container is tightly sealed to prevent glycerol evaporation, which may cause specimen dehydration and shrinkage.
3. This product is intended only for final preservation of teratogenicity skeletal specimens and must not be used for preliminary soft-tissue clearing or staining procedures.
4. If fungal filaments, turbidity, or suspended matter appear in the preservation solution, promptly replace it with fresh preservation solution.
5. Complete supporting procedure: Bone Tissue Clearing Solution (KOH Solution) for Teratogenicity Testing (B1529929) → Alizarin Red skeletal staining → Bone Tissue Clearing Solution A (KOH-Glycerol Solution) for Teratogenicity Testing (B1529923) → Bone Tissue Clearing Solution B (Glycerol Solution) for Teratogenicity Testing (B1529922) → long-term preservation in this product; the other half of the fetuses may be treated with Fixative Solution for Teratogenicity Testing (Visceral Examination) (T1529295) for visceral teratogenicity examination.
6. For your safety and health, please wear a laboratory coat and disposable gloves during operation.
规格
规格或纯度
BioReagent, 组织学级, 用于显微镜
稳定性与储存
Store at room temperature long term (12 months).
英文名称
Bone Tissue Preservation Solution for Teratogenicity Testing
储存条件
室温
运输条件
常规运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
高级数据
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
需要帮助选择级别？
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Histological grade 级别指南 → 查看 for Microscopy 级别指南 →产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 SSE:688179 保留所有权利。
危险品化学品经营许可证（带存储）