计算溶液所需的质量、体积或浓度。
BioReagent,分子生物学级,无菌 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
λ 噬菌体是最早应用的克隆载体之一,基因组为约 50 kb 的双链 DNA。λ 噬菌体在宿主细胞内存在两种增殖途径:①裂解生长:环状噬菌体 DNA 在宿主细胞内大量复制,合成噬菌体相关蛋白并组装为成熟噬菌体颗粒,最终裂解宿主菌,释放子代噬菌体继续侵染;②溶源性生长:λ 噬菌体 DNA 整合至宿主染色体 DNA 上,随宿主基因组同步复制并传递给子代细胞,宿主细胞不发生裂解。科研中常利用 λ 噬菌体的裂解生长特性,扩增制备高滴度噬菌体颗粒,并用于 λ 噬菌体 DNA 提取。
噬菌体 SM 缓冲液主要由硫酸镁、氯化钠、明胶等组分配制而成,经高压灭菌处理,为无菌溶液,适用于 λ 噬菌体原液的稀释与保存。 本试剂仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤(仅供参考):
1、根据实验具体要求操作。
2、由于该试剂含有明胶,在低温下有可能形成胶冻状,可25~37℃孵育直至完全溶解,充分混匀后使用。
注意事项:
1、注意无菌操作,尽量避免污染。
2、试剂开封后请尽快使用,以防影响后续实验效果。
3、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Bacteriophage λ is one of the earliest cloning vectors. Its genome is a double-stranded DNA of approximately 50 kb. Bacteriophage λ exhibits two life cycles within host cells: ① Lytic growth: the circular phage DNA replicates extensively in the host cell, synthesizes phage gene products and assembles into mature phage particles. Eventually, the host cell is lysed to release progeny phages for subsequent infection. ② Lysogenic growth: λ phage DNA integrates into the host chromosomal DNA and replicates together with the host genome, passing on to daughter cells without lysis of the host. In research, the lytic cycle of bacteriophage λ is commonly utilized to propagate high-titer phage particles for λ phage DNA extraction.
Bacteriophage SM Buffer consists mainly of magnesium sulfate, sodium chloride and gelatin. It is autoclaved to obtain a sterile solution, and is used for dilution and storage of bacteriophage λ stocks. For research use only. Not for clinical diagnosis or other applications.
Procedure (For reference only):
1. Operate according to specific experimental requirements.
2. Since this reagent contains gelatin, it may form a gel-like substance at low temperatures. Incubate at 25–37 °C until completely dissolved, mix thoroughly before use.
Precautions:
1. Maintain aseptic technique to avoid contamination.
2. Use the reagent promptly after opening to prevent compromised experimental performance.
3. For your safety and health, wear a lab coat and disposable gloves during operation.
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