计算溶液所需的质量、体积或浓度。
,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
5-溴,4-氯,3-吲哚磷酸酯(BCIP)/硝基蓝四氮唑(NBT)底物,用于定位碱性磷酸酶(AP)标记的探针。BCIP充当AP的底物,并在去磷酸化后进一步反应,得到深蓝色靛蓝染料作为氧化产物。NBT在本文中用作氧化剂,还提供深蓝色染料,增强了颜色并使检测更灵敏。
5-bromo, 4-chloro, 3-indolylphosphate (BCIP)/Nitro-Blue Tetrazolium (NBT) substrate, used for the localization of alkaline phosphatase (AP) labeled probes on Western, Northern, Southern and dot blots. BCIP acts as the substrate for AP and reacts further after the dephosphorylation to give a dark-blue indigo-dye as an oxidation product; NBT serves herein as the oxidant and also gives a dark-blue dye, intensifying the color and making the detection more sensitive.|Recommended for use with Crystal Mounting Medium (sc-24943).
Preparation of staining solution:•Add 200 µl of BCIP/NBT stock solution to the following room temperature buffer: 10 ml 0.1 M Tris Hydrochloride, pH 9.5, 0.1 M Sodium Chloride, and 0.05 M Magnesium Chloride. (do not include Magnesium Chloride in the detec
BCIP/NBT Stock Solution, 50Xis a substrate for alkaline phosphatase (AP) based detection systems including western blotting
P264: 处理后要彻底洗手。