计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Product Description
Beta Galactosidase from Streptococcus pneumoniae releases only β(1-4)- linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins. β(1-4) galactose is by far the most common linkage found in N-linked oligosaccharides. For other galactosidase linkages, ß(1-3,4,6)-Galactosidase from Bovine testes is recommended. The enzyme is as active on tetraantennary oligosaccharides as on disaccharides containing β(1-4)-linked galactose. Fucose linked to the penultimate N-acetylglucosamine will block cleavage of the galactose. Up to 100 υg of asialofetuin can be completely β(1-4)-degalactosylated in less than 1 hour using 3 mU of enzyme.
Contents
ß-(1-4) Galactosidase in 20 mM Tris-HCl, 25 mM NaCl (pH 7.5).
Included with 20 µL and 60 µL pack sizes:
5x Reaction Buffer 6.0 (250 mM sodium phosphate, pH 6.0).
Molecular Weight
~250,000 daltons
pH optimum
6.0, active over the range 5-7.
The supplied buffer concentrate provides the optimal pH for enzyme activity with the standard substrate. If glycosidase treatment is performed at suboptimal pH because of glycoprotein solubility or activity requirements, expect some diminution in enzyme activity.
Formulation
The enzyme is provided as a sterile-filtered solution in 20 mM Tris-HCl, 25 mM NaCl (pH 7.5).
Specific Activity
One unit of ß-(1-4)-galactosidase is defined as the amount of enzyme required to produce 1 µmole of p-nitrophenol (pNP) in 1 minute at 37°C pH 5 from p-nitrophenyl-beta-D-galactopyranoside.
Specificity
Non-reducing terminal ß(1-4)-Galactose. Number of antennae does not affect cleavage rate. Fucose linked to the penultimate N-acetylglucosamine will block cleavage of the galactose.
Stability
Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity.
Purity
ß(1-4)-Galactosidase is tested for contaminating protease as follows: 10 µg of denatured BSA is incubated at 37°C for 24 hours with 2 µl of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation.
The production host strain has been extensively tested and does not produce any detectable glycosidases.
Directions for use
1. Add up to 100 µg of asialoglycoprotein or 1 nmol of oligosaccharide to tube.
2. Add deionized water to a total of 14 µl.
3. Add 4 µl of 5x Reaction Buffer 6.0.
4. Add 2 µl ß(1-4) Galactosidase.
5. Incubate at 37°C for 1 hour.
For glycoproteins, cleavage may be monitored by SDS-PAGE if the size differential between native and de-galactosylated protein is sufficient for detection.
Note: The optimum pH for cleavage of oligosaccharides is ~6.
规格
产品名称
Beta Galactosidase
别名
β-半乳糖苷酶
规格或纯度
Specific Activity ＞6 U/mg;Activity ＞3 U/ml
生物活性
>3 U/ml
酶学委员会编号
3.2.1.23
分子类型
酶
产品线
生物产品
储存与运输
浓度
Specific Activity ＞6 U/mg;Activity ＞3 U/ml
储存条件
2-8°C储存
运输条件
低温运输
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）