计算溶液所需的质量、体积或浓度。
概述
Product Description
Beta-(1-3,4,6) Galactosidase cleaves all β(1-3) and β(1-4) linked non-reducing, terminal galactose. β(1-6) linked galactose is released at a slower rate. The enzyme is a glycoprotein.
β(1-4) galactose is by far the most common linkage found in N-linked oligosaccharides. For cleavage of this linkage we recommend β(1-4) Galactasidase.
Contents
200 µls of Beta-(1-3,4,6) Galactosidase in 20 mM Tris-HCl, 50 mM NaCl, 0.5mg/ml BSA,pH 7.5
5x Reaction Buffer- 500 mM sodium citrate/phosphate pH 4
Optimum pH 4
The supplied buffer concentrate provides the optimal pH for enzyme activity with the standard substrate. If glycosidase treatment is performed at suboptimal pH because of glycoprotein solubility or activity requirements, expect some diminution in enzyme activity.
Specific Activity
One unit of ß-(1-3,4,6)-Galactosidase is defined as the amount of enzyme required to produce 1 µmole of p-nitrophenol (pNP) in 1 minute at 37°C, pH 4.0 from p-nitrophenyl-ß-D-galactopyranoside.
Specificity
Cleaves all ß1-3 and ß1-4 linked non-reducing, terminal galactose. ß1-6 linked galactose is released at a slower rate.
Formulation
The enzyme is provided as a sterile-filtered solution in 20 mM Tris-HCl pH 7.5, 50 mM NaCl, 0.5 mg/ml BSA, pH 7.5.
Stability
Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity.
Purity
ß-(1-3,4,6)-Galactosidase is tested for contaminating protease as follows: 10 µg of denatured BSA is incubated at 37°C for 24 hours with 2 µl of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation.
Each lot is also tested for contaminating activities by incubating the enzymes with the appropriate substrates for 24 hours; the detection limit is 5 µU/ml (IUB). A passing lot will have no detectable activity.
Directions for use
1. Add up to 100 µg of asialoglycoprotein or 1 nmol of oligosaccharide to tube.
2. Add deionized water to a total of 14 µl.
3. Add 4 µl of 5x Reaction Buffer 4.
4. Add 2 µl ß-Galactosidase.
5. Incubate at 37°C for 1 hour.
For glycoproteins, cleavage may be monitored by SDSPAGE if the size differential between native and degalactosylated protein is sufficient for detection
规格
产品名称
Beta-(1-3,4,6) Galactosidase
别名
β-(1-3,4,6) 半乳糖苷酶
规格或纯度
Specific Activity >10.2 U/mg;Activity >2.5 U/ml
生物活性
>2.5 U/ml
分子类型
酶
产品线
生物产品
储存与运输
浓度
Specific Activity ＞10.2 U/mg;Activity ＞2.5 U/ml
储存条件
-20°C储存
运输条件
超低温运输
高级数据
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
常见问题
本产品应如何储存？
请于?20 °C条件下保存。需冷冻保存以维持规定的有效期。
本产品如何运输？
本产品采用保温箱配冰袋运输。收货后请立即拆包，并按上述储存条件保存。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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