计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,干燥。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Product introduction：
BS3-d0/d4 reagents form a matched set of heavy (deuterated) and light mass varieties of BS3 amine-reactive crosslinker that facilitates the identification of crosslinked peptides by mass spectrometry.
The BS3-d0/d4 reagents are mass-analogs of the popular BS3 crosslinker (). The d-zero variety (BS3-d0) possesses ordinary hydrogen atoms while the d-four variety (BS3-d4) contains four deuterium atoms that make it exactly four hydrogen mass units heavier. When the two varieties are mixed and used to crosslink interacting proteins, distinctive mass patterns result that enable unambiguous identification of the crosslinked molecules.
Features of BS3-d0/d4 reagents:
• Deuterium-labeled BS3 and its standard hydrogen-only analog—specially manufactured for crosslinking protein structure:function analysis by mass spectrometry
• Easy identification of crosslinked fragments—peptide masses differing by 4 units unambiguously identify crosslink at specific loci within a protein interaction structure
• Application-specific instructions—help guide the first time user through the application
• Method requires only microgram amounts of protein—excellent alternative to NMR or X-ray crystallography-based methods that require large amounts of protein, special solvents or crystal formation
Addition of an equimolar mixture of two identical crosslinking agents that differ only in the number of deuterium atoms in their chemical structures provides a powerful method to identify low-abundance, singly-crosslinked peptides by mass spectrometry. Crosslinking protein interactions with BS2G-d0/d4 reagents generates distinctive mass patterns in which crosslinked peptides differ by 4 mass units after enzymatic digestion of the crosslinked protein or protein complex. Further analysis of the reaction products can yield low resolution three dimensional structure information.
BS3-d0/d4 andBS2G-d0/ reagents are homobifunctional, amine-reactive, non-cleavable, water-soluble crosslinking agents with defined spacer arm lengths. These reagents can act as molecular rulers for estimation of spatial relationships in protein structure-function studies. Both light and deuterated analogs react efficiently with primary amine (—NH2) at pH 7-9 to form stable amide bonds. Proteins generally contain several primary amines in the side chain of Lysine (K) residues and the N-terminus of each polypeptide that are available as targets for the two sulfo-NHS ester reactive groups found in each of these reagents. These reagents are supplied as a sodium salt and are soluble in water at a concentration up to 10 mM.
• Reactive Moiety:Sulfo-NHS Ester
• Crosslinker Type:Homobifunctional
• Form:Powder
• Spacer:Medium(10 to 30 Å)
• Labeling Method:Chemical Labeling
• Spacer Arm Length:11.4 Å
The BS3-d0/d4 reagents are mass-analogs of the popular BS3 crosslinker (). The d-zero variety (BS3-d0) possesses ordinary hydrogen atoms while the d-four variety (BS3-d4) contains four deuterium atoms that make it exactly four hydrogen mass units heavier. When the two varieties are mixed and used to crosslink interacting proteins, distinctive mass patterns result that enable unambiguous identification of the crosslinked molecules.
Features of BS3-d0/d4 reagents:
• Deuterium-labeled BS3 and its standard hydrogen-only analog—specially manufactured for crosslinking protein structure:function analysis by mass spectrometry
• Easy identification of crosslinked fragments—peptide masses differing by 4 units unambiguously identify crosslink at specific loci within a protein interaction structure
• Application-specific instructions—help guide the first time user through the application
• Method requires only microgram amounts of protein—excellent alternative to NMR or X-ray crystallography-based methods that require large amounts of protein, special solvents or crystal formation
Addition of an equimolar mixture of two identical crosslinking agents that differ only in the number of deuterium atoms in their chemical structures provides a powerful method to identify low-abundance, singly-crosslinked peptides by mass spectrometry. Crosslinking protein interactions with BS2G-d0/d4 reagents generates distinctive mass patterns in which crosslinked peptides differ by 4 mass units after enzymatic digestion of the crosslinked protein or protein complex. Further analysis of the reaction products can yield low resolution three dimensional structure information.
BS3-d0/d4 andBS2G-d0/ reagents are homobifunctional, amine-reactive, non-cleavable, water-soluble crosslinking agents with defined spacer arm lengths. These reagents can act as molecular rulers for estimation of spatial relationships in protein structure-function studies. Both light and deuterated analogs react efficiently with primary amine (—NH2) at pH 7-9 to form stable amide bonds. Proteins generally contain several primary amines in the side chain of Lysine (K) residues and the N-terminus of each polypeptide that are available as targets for the two sulfo-NHS ester reactive groups found in each of these reagents. These reagents are supplied as a sodium salt and are soluble in water at a concentration up to 10 mM.
• Reactive Moiety:Sulfo-NHS Ester
• Crosslinker Type:Homobifunctional
• Form:Powder
• Spacer:Medium(10 to 30 Å)
• Labeling Method:Chemical Labeling
• Spacer Arm Length:11.4 Å
规格
别名
BS3-d4(双(磺化琥珀酰亚胺基)2,2,7,7-辛二酸酯-d4)
英文名称
BS3-d4(bis(sulfosuccinimidyl) 2,2,7,7-suberate-d4)
储存条件
2-8°C储存,干燥
运输条件
低温运输
名称和识别符
分子类型
小分子
分子量
576.45
技术文档
高级数据
产品属性
| Reactivity | Amine-Amine |
|---|
化学和物理性质
溶解性
Water
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）