胆固醇酯酶 (CHE)

Name: 胆固醇酯酶 (CHE)
SKU: C1492993
Availability: InStock

CAS: 9026-00-0 货号: C1492993 EC号: 232-808-6

5篇引用文献 COA

有货

级别和纯度: 生物活性 ? 重组 ? ActiBioPure™ ? 高性能 ? EnzymoPure™ ? 100-250 U/mg enzyme powder; ≥100 U/mg protein

生物活性

100-250 U/mg enzyme powder; ≥100 U/mg protein

规格

库存

价格

数量

1KU

C1492993-1KU

现货

¥599.90

5KU

C1492993-5KU

现货

¥1,799.90

25KU

C1492993-25KU

现货

¥4,799.90

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概述规格储存与运输技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 此产品的引用文献技术文档和文章溶液计算器

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为什么选择此级别

ActiBioPure™,EnzymoPure™,生物活性,重组,高性能级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

避光,-20°C储存,干燥,避免反复冻融。超低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 5 篇同行评审文献引用。

概述

Cholesterol Esterase from Pseudomonas sp. is an enzyme that hydrolyzes cholesterol ester to cholesterol and free fatty acid in the intestinal lumen. Cholesterol synthesized in the acinar cells and is stored in zymogen granules. Cholesterol esterase is also known as bile salt-stimulated lipase and carboxy ester lipasea, acts function for acceleration of cholesterol absorption.

Cholesterol ester + H₂O → Cholesterol + Fatty acid

Preparation and Specification

Appearance	: White to pale brownish amorphous powder, lyophilized
Activity	: ≥100U/mg powder

Properties

Molecular weight	30 kDa (SDS-PAGE)
Isoelectric point	pH 5.28 (estimated from amino acid sequence)
Optimum pH	6.5
pH Stability	6.0–11.0 (37℃, 60 min, in 0.1% BSA)
Optimum temperature	35–40℃ (Phosphate Buffer)
Thermal stability	Stable at 45℃ and below (pH 8.0, 30 min)
Storage stability	At least one year at -20℃.
Activator	Adekatol TN-100, Adekatol PC-8, Adelatol SO-120, Adekatol SO-135, Triton X-100

Applications

This enzyme is useful for enzymatic determination of total cholesterol, HDL-C, and LDL-C coupled with cholesterol oxidase.

ASSAY

Principle:

The assay is based on the increase in absorbance at 493 nm as the formation of quinoneimine dye proceeds in the following reactions:

Unit definition:

One unit is defined as the amount of enzyme which liberates 1 μmole of cholesterol per minute at 37°C under the conditions specified in the assay procedure.

Reagents

1. Reaction mixture

0.2M KH₂PO₄-NaOH buffer, pH 6.8	0.60 ml
0.35% (W/V) 4-AA solution	0.30 ml
0.2% (W/V) Phenol solution	0.30 ml
100 U/ml HRP (Horseradish Peroxidase) solution <sup>①</sup>	0.30 ml
3% (W/V) Triton X-100 solution	0.30 ml
0.2 U/ml CHO (Cholesterol Oxidase) solution <sup>②</sup>	0.60 ml
Substrate solution <sup>③</sup>	0.30 ml
Distilled water	0.30 ml

① 100 U/ml HRP solution: Dissolve 1000 U (PPU) of HRP with 10 ml of distilled water.

② 0.2 U/ml CHO solution: Dissolve 2 U of CHO with CHO dilution buffer <sup>※</sup>

※ CHO dilution buffer: 0.1 M KH₂PO₄-Na₂HPO₄ buffer, pH 7.0containing 0.05% (W/V) Triton X-100.

③ Substrate solution: Calf serum.

2. Enzyme dilution buffer: 10 mM KH₂PO₄–NaOH buffer pH 7.5 containing 0.1% (W/V) bovine serum albumin (BSA).

Enzyme solution

Accurately weigh about 20 mg of the sample and add enzyme dilution buffer to make a total of 20 ml. Dilute it with enzyme dilution buffer to adjust the concentration to within 0.3–0.5 U/ml.

Procedure

1. Pipette accurately 3.0 ml of reaction mixture into a small test tube and preincubate it at 37°C.

2. After 10 min, add 50 μl of enzyme solution and mix to start the reaction at 37°C.
※ In the case of a test blank, add 50 μl of enzyme dilution buffer in place of enzyme solution.

3. After starting the reaction, measure the rate of increase per minute in absorbance at 493 nm. The rate must be measured within the linear portion of the absorbance curve.

Absorbance sample :	As/min
blank :	Ab/min

△A/min =(As/min−Ab/min)≤0.050 Abs/min

Calculation

Activity(U/mg of powder)= {(△A/min)/(12.0×1/2)} × 3.05/0.05 × 1/x

12.0 :	millimolar extinction coefficient of quinoneimine dye at 493 nm(cm²/μmole)
1/2 :	a multiplier derived from the fact that 2 mole of H2O2 produce 1 mole of quinoneimine dye
3.05 :	final volume(ml)
0.05 :	volume of enzyme solution(ml)
X :	concentration of the sample in enzyme solution(mg/ml)

规格

产品名称

胆固醇酯酶 (CHE)

别名

英文别名

规格或纯度

生物活性, 重组, ActiBioPure™, 高性能, EnzymoPure™, 100-250 U/mg enzyme powder; ≥100 U/mg protein

生物活性

100-250 U/mg enzyme powder; ≥100 U/mg protein

种属

Pseudomonas sp.

来源

重组表达

CAS编号和信息

9026-00-0

酶学委员会编号

EC 3.1.1.13

分子类型

酶

产品线

生物产品

储存与运输

物理形态

冻干(Lyophilized)

浓度

100-250 U/mg enzyme powder; ≥100 U/mg protein

复溶

Reconstitute in 10 mM KH2PO4–NaOH buffer pH 7.5, containing 0.1%(W/V) BSA.

储存条件

避光,-20°C储存,干燥,避免反复冻融

运输条件

超低温运输

稳定性与储存

长期储存-20℃（12个月）；复溶后建议分装，避免反复冻融。避光；密封。

单位定义

One unit is defined as the amount of enzyme which liberates 1 μmole of cholesterol per minute at 37°C under the conditions specified in the assay procedure.