计算溶液所需的质量、体积或浓度。
BioReagent,即用型,无菌,用于细胞培养 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
是否无菌:是
消化终止液是阿拉丁自主研发出的用于胰酶消化细胞时,终止消化的一类试剂。胰酶消化液中含有的胰蛋白酶是一种丝氨酸蛋白酶,本品中含有抗凝血酶III,是一种丝氨酸蛋白酶抑制剂,因此对胰酶的消化细胞起到抑制作用。本消化终止液经过无菌处理,可直接用于细胞实验。
使用说明(贴壁细胞):
a. 去除培养液后用无菌PBS轻轻洗涤细胞一次以除去残留的血清。
b. 加入适量的胰酶细胞消化液消化细胞,(一般25cm²细胞培养瓶加入1ml胰酶消化液)。
c. 显微镜下观察,细胞明显收缩,并且肉眼观察培养器皿底部发现细胞的形态发生明显的变化,消化时间在30s-3min内,不宜过长。
d. 立即加入与胰酶等量(1ml)的消化终止液,随后把细胞轻轻吹打下来,并适当吹散和重悬。
注:千万不要过度消化细胞,以消化至刚好能把细胞吹打下来为最佳。消化过度的细胞由于后续生长状况会比较差,通常不宜再用于冷冻保存。吹打和重悬细胞过程需要适当轻柔,否则可能会影响复苏时的细胞存活率。
e. 收集细胞或进行后续试验。
注意事项:
1. 在使用消化终止液的过程中要特别注意,避免终止液的污染。
2. 如果每次用量不大,建议分装后使用。
3. 本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
4. 为了您的安全和健康,请穿实验服并戴一次性手套操作。
Is it sterile: Yes
Digestion Termination Solution is a type of reagent independently developed by Aladdin, designed to terminate digestion when cells are digested with trypsin. Trypsin contained in trypsin digestion solution is a serine protease, while this product contains antithrombin III, a serine protease inhibitor. Therefore, it exerts an inhibitory effect on the cell digestion process mediated by trypsin. This Digestion Termination Solution has undergone sterile treatment and can be directly used in cell experiments.
Instructions for Use (Adherent Cells):
a. After removing the culture medium, gently wash the cells once with sterile PBS to eliminate residual serum.
b. Add an appropriate amount of trypsin cell digestion solution to digest the cells (typically, 1 mL of trypsin digestion solution is added to a 25 cm² cell culture flask).
c. Observe under a microscope: when the cells shrink significantly and obvious changes in cell morphology are visible to the naked eye at the bottom of the culture vessel, stop digestion. The digestion time should be between 30 seconds and 3 minutes, and should not be prolonged.
d. Immediately add an equal volume (1 mL) of Digestion Termination Solution to the trypsin. Subsequently, gently pipette to detach the cells, then properly disperse and resuspend them.
Note: Do not over-digest the cells. The optimal digestion endpoint is when the cells can just be detached by pipetting. Over-digested cells usually cannot be used for cryopreservation, as their subsequent growth performance will be poor. The process of pipetting and resuspending the cells must be appropriately gentle; otherwise, it may affect the cell survival rate during thawing.
e. Collect the cells or proceed with subsequent experiments.
Precautions:
1. Special attention should be paid during the use of the Digestion Termination Solution to avoid contamination of the solution.
2. If the amount used each time is small, it is recommended to aliquot the product before use.
3. This product is intended solely for scientific research by professional personnel. It shall not be used for clinical diagnosis or treatment, nor for food or pharmaceutical purposes, and shall not be stored in ordinary residential premises.
4. For your safety and health, please wear a lab coat and disposable gloves during operation.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | C1501908 |
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