计算溶液所需的质量、体积或浓度。
BioReagent,用于显微镜,用于细胞培养 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
| 项目号 | Component | 50T | Storage |
| C1506003A | 结晶紫染色液 | 50 mL | 2-8℃. Store in the dark. |
| C1506003B | 调色液 | 50 mL | 2-8℃ |
| C1506003C | 封片液 | 5 mL | 2-8℃ |
操作步骤:
样品处理:
a)石蜡切片:
1. 二甲苯中脱蜡5-10分钟。
2. 换用新鲜的二甲苯,再脱蜡5-10分钟。
3. 无水乙醇5分钟。
4. 90%乙醇2分钟。
5. 70%乙醇2分钟。
6. PBS洗,2分钟,3次。
7. 结晶紫染色液10分钟。
8. 用C液封片。
注意:如果颜色不深,可以增加染色时间;如果颜色过深,可以用B液洗,每次30秒到一分钟,后用水洗。
b)冰冻切片:
1. 用4%多聚甲醛固定30分钟。
2. PBS洗3次每次5分钟。
3. 结晶紫染色液10分钟。
4. 用C液封片。
注意:如果颜色不深,可以增加染色时间;如果颜色过深,可以用B液洗,每次30秒到一分钟,后用水洗。
c)培养细胞:
1. 用4%多聚甲醛固定10分钟。
2. PBS洗3次每次5分钟。
3. 结晶紫染色液10分钟。
4. 用C液封片
注意:如果颜色不深,可以增加染色时间;如果颜色过深,可以用B液洗,每次30秒到一分钟,后用水洗。
注意事项:
1.第一次使用本试剂时建议先取1-2个样品做预实验,确定一个好染色时间。
2.为了您的安全和健康,请穿实验服并戴一次性手套操作。
| C1506003 | Component | 50T | Storage |
| C1506003A | Crystal Violet Staining Solution | 50 mL | 2-8℃. Store in the dark. |
| C1506003B | Toning Solution | 50 mL | 2-8℃ |
| C1506003C | Mounting Medium | 5 mL | 2-8℃ |
Operating Procedures:
Sample Processing:
a) Paraffin Sections:
1. Dewax in xylene for 5–10 minutes.
2. Replace with fresh xylene and dewax again for 5–10 minutes.
3. Immerse in absolute ethanol for 5 minutes.
4. Immerse in 90% ethanol for 2 minutes.
5. Immerse in 70% ethanol for 2 minutes.
6. Rinse with PBS for 2 minutes, repeated 3 times.
7. Stain with Crystal Violet Staining Solution for 10 minutes.
8. Mount the section with Solution C.
Note: If the staining color is too light, extend the staining time. If the staining color is too dark, rinse with Solution B (30 seconds to 1 minute each time), followed by rinsing with water.
b) Frozen Sections:
1. Fix with 4% paraformaldehyde for 30 minutes.
2. Rinse with PBS 3 times, 5 minutes each time.
3. Stain with Crystal Violet Staining Solution for 10 minutes.
4. Mount the section with Solution C.
Note: If the staining color is too light, extend the staining time. If the staining color is too dark, rinse with Solution B (30 seconds to 1 minute each time), followed by rinsing with water.
c) Cultured Cells:
1. Fix with 4% paraformaldehyde for 10 minutes.
2. Rinse with PBS 3 times, 5 minutes each time.
3. Stain with Crystal Violet Staining Solution for 10 minutes.
4. Mount with Solution C.
Note: If the staining color is too light, extend the staining time. If the staining color is too dark, rinse with Solution B (30 seconds to 1 minute each time), followed by rinsing with water.
Precautions:
1. When using this reagent for the first time, it is recommended to take 1-2 samples for a preliminary experiment to determine an optimal staining time.
2. For your safety and health, please wear a lab coat and disposable gloves during operation.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | C1506003 | |
| 分析证书 | C1506003 |
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