储存缓冲液：Purified Water, 0.05% ProClin 300
阿拉丁羧基乳胶微球是通过乳液聚合制备得到的聚苯乙烯微球，尺寸范围80~350 nm，表面修饰有适量的羧基基团，可方便地与抗体或带有氨基的活性物质进行共价偶联，得到稳定的微球-配基复合物，可广泛应用于粒子增强免疫比浊试剂产品。
| 产品名称 | 货号 | 粒径 | 浓度 | 羧基含量参考值 | PA参考值 | 应用方向 |
| 羧基乳胶微球 | C1506724-80nm | 80nm | 10% | 310 | 34 | 免疫比浊 |
| 羧基乳胶微球 | C1506724-100nm | 100nm | 10% | 220 | 39 | 免疫比浊 |
| 羧基乳胶微球 | C1506724-120nm | 120nm | 10% | 210 | 34 | 免疫比浊 |
| 羧基乳胶微球 | C1506724-140nm | 140nm | 10% | 190 | 32 | 免疫比浊 |
| 羧基乳胶微球 | C1506724-160nm | 160nm | 10% | 160 | 34 | 免疫比浊 |
| 羧基乳胶微球 | C1506724-180nm | 180nm | 10% | 150 | 30 | 免疫比浊 |
| 羧基乳胶微球 | C1506724-200nm | 200nm | 10% | 130 | 32 | 免疫比浊 |
| 羧基乳胶微球 | C1506724-250nm | 250nm | 10% | 75 | 48 | 免疫比浊 |
| 羧基乳胶微球 | C1506724-300nm | 300nm | 10% | 85 | 33 | 免疫比浊 |
| 羧基乳胶微球 | C1506724-350nm | 350nm | 10% | 60 | 45 | 免疫比浊 |
| 名称 | 详情 |
| 反应缓冲液 | 50mM PB, pH 7.0 |
| EDC溶液 | 10mg/mL in PB, pH 6.0 |
| 封闭液 | 50mM PB, pH 7.4, 1% BSA |
| 保存液 | 50mM Tris-HCl, pH 8.2 |
| 名称 | 详情 |
| 反应缓冲液 | 50mM PB, pH 7.0 |
| EDC溶液 | 10mg/mL in PB, pH 6.0 |
| 封闭液 | 50mM PB, pH 7.4, 1% BSA |
| 保存液 | 50mM Tris-HCl, pH 8.2 |
Aladdin Carboxyl Latex Beads are polystyrene microspheres prepared by emulsion polymerization, with a size range of 80–350 nm. The surface is modified with an appropriate amount of carboxyl groups, allowing convenient covalent conjugation with antibodies or other active substances containing amino groups, resulting in stable microsphere-ligand complexes. They are widely applicable in particle-enhanced immunoturbidimetric assay reagents.
| Catalog Number | Particle Size | Concentration | Carboxyl Content Reference Value (μeq/g) | PA Reference Value | Application |
| C1506724-80nm | 80nm | 10% | 310 | 34 | Immunoturbidimetry |
| C1506724-100nm | 100nm | 10% | 220 | 39 | Immunoturbidimetry |
| C1506724-120nm | 120nm | 10% | 210 | 34 | Immunoturbidimetry |
| C1506724-140nm | 140nm | 10% | 190 | 32 | Immunoturbidimetry |
| C1506724-160nm | 160nm | 10% | 160 | 34 | Immunoturbidimetry |
| C1506724-180nm | 180nm | 10% | 150 | 30 | Immunoturbidimetry |
| C1506724-200nm | 200nm | 10% | 130 | 32 | Immunoturbidimetry |
| C1506724-250nm | 250nm | 10% | 75 | 48 | Immunoturbidimetry |
| C1506724-300nm | 300nm | 10% | 85 | 33 | Immunoturbidimetry |
| C1506724-350nm | 350nm | 10% | 60 | 45 | Immunoturbidimetry |
Product Features
Uniform particle size with low batch-to-batch variation.
Single-batch production capacity up to 100 L, meeting most production demands.
Customized services are available.
Product Parameters
Material: Polystyrene copolymer
Solid Content: 10% w/v
Particle Size Range: 80–350 nm
Uniformity: CV ≤ 3%
Dispersion Medium: Purified Water, 0.05% ProClin 300
Instructions for Use
One-Step Labeling Protocol
1. Solution Preparation
| Name | Details |
| Reaction Buffer | 50mM PB, pH 7.0 |
| EDC Solution | 10mg/mL in PB, pH 6.0 |
| Blocking Buffer | 50mM PB, pH 7.4, 1% BSA |
| Storage Buffer | 50mM Tris-HCl, pH 8.2 |
2. Recommended Labeling Protocol
(1) Ultrasonic dispersion is recommended before use.
(2) Dilute the microspheres 10-fold to 1% (w/v) using Reaction Buffer.
(3) Add 0.2–0.8 mg of antibody per mL of microspheres. Mix well.
(4) Add 20 μL of EDC Solution. Mix well.
(5) React on a mixer for 2 hours.
(6) Replace the solution twice with 50 mM PB, pH 7.4. Add 1 mL of Blocking Buffer and react for 1 hour.
(7) Replace the solution twice with Storage Buffer. Resuspend in 1 mL of Storage Buffer and store at 2–8°C.
Two-Step Labeling Protocol
1. Solution Preparation
| Name | Details |
| Reaction Buffer | 50mM PB, pH 7.0 |
| EDC Solution | 10mg/mL in PB, pH 6.0 |
| Blocking Buffer | 50mM PB, pH 7.4, 1% BSA |
| Storage Buffer | 50mM Tris-HCl, pH 8.2 |
2. Recommended Labeling Protocol
(1) Ultrasonic dispersion is recommended before use.
(2) Dilute the microspheres 10-fold to 1% (w/v) using Reaction Buffer.
(3) Add 20 μL of EDC Solution. Mix well and react on a mixer for 15 minutes.
(4) Replace the solution twice with Reaction Buffer.
(5) After dispersing the microspheres in 1 mL of Reaction Buffer, add 200–800 μg of antibody. Mix well and react on a mixer for 2 hours.
(6) Replace the solution twice with 50 mM PB, pH 7.4. Add 1 mL of Blocking Buffer and react for 1 hour.
(7) Replace the solution twice with Storage Buffer. Resuspend in 1 mL of Storage Buffer and store at 2–8°C.
Precautions
1. This product series should be stored in a cool, sealed environment. Do not freeze.
2. Before use, disperse the microspheres evenly using ultrasonication or a vortex mixer to ensure uniform dispersion.
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ25F1230321 |
分析证书 | C1506724 |
| ZJ25F1230322 |
分析证书 | C1506724 |
| ZJ25F1230323 |
分析证书 | C1506724 |
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