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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
阿拉丁研发生产的ChIP-Grade Protein A/G琼脂糖磁珠,英文全称ChIP-Grade Protein A/G Magnetic Agarose Beads,别名包括ChIP级Protein A/G磁珠、ChIP级Protein A/G免疫沉淀磁珠、ChIP级蛋白A/G磁珠、ChIP级蛋白A/G免疫沉淀磁珠,是科研实验中分离免疫复合物的优质工具。
本产品采用高质量重组Protein A与Protein G与琼脂糖磁珠共价偶联,经特殊工艺处理,可高特异性结合相应抗体,对蛋白、DNA及RNA的非特异性吸附极低,能有效避免实验干扰,保障实验准确性。主要适用于染色质免疫沉淀(ChIP)、RNA免疫沉淀(RIP)等实验中免疫复合物的分离与富集。
产品操作便捷高效,可借助磁力架等磁分离设备,快速沉淀与目的蛋白结合的染色质片段或RNA片段;储存在专用特殊保护液中,不含甘油,通过磁性吸附即可实现快速分离,无需离心操作,大幅简化实验流程,提升实验效率,适配各类科研实验场景需求。
使用说明:
1. ChIP-Grade Protein A+G 琼脂糖磁珠可用于ChIP及RIP等实验。
2. 单次检测使用约50-80µl ChIP-Grade Protein A+G琼脂糖磁珠。
3. 磁性分离:置于磁力架上分离10秒,去除上清即可。
注意事项:
1. 本产品需维持pH为6-8,避免高速离心、干燥;请勿长时间将磁珠置于磁场中,否则可能会引起磁珠聚团。
2. 本产品使用前要适当充分重悬,即颠倒若干次使磁珠混合均匀,混匀操作须轻柔,不宜剧烈涡旋震荡等,避免蛋白变性。
3. 如果使用真空泵等仪器吸取上清液,须注意真空泵的吸液强度,以免吸力过大而吸取到聚集的磁珠。
Background Introduction:
The ChIP-Grade Protein A/G Magnetic Agarose Beads, developed and produced by Aladdin, with the full English name "ChIP-Grade Protein A/G Magnetic Agarose Beads," are also referred to as ChIP-grade Protein A/G magnetic beads, ChIP-grade Protein A/G immunoprecipitation magnetic beads, ChIP-grade Protein A/G beads, or ChIP-grade Protein A/G immunoprecipitation beads. They serve as an excellent tool for isolating immune complexes in scientific research experiments.
This product is prepared by covalently coupling high-quality recombinant Protein A and Protein G with magnetic agarose beads. Through specialized processing, it exhibits high specificity for binding to corresponding antibodies while maintaining extremely low non-specific adsorption to proteins, DNA, and RNA, effectively minimizing experimental interference and ensuring accuracy. It is primarily suitable for the isolation and enrichment of immune complexes in experiments such as Chromatin Immunoprecipitation (ChIP) and RNA Immunoprecipitation (RIP).
The product offers convenient and efficient operation. With the aid of magnetic separation equipment such as magnetic racks, it enables rapid precipitation of chromatin fragments or RNA fragments bound to target proteins. Stored in a dedicated special protective solution without glycerol, it allows quick separation via magnetic adsorption, eliminating the need for centrifugation. This significantly simplifies experimental procedures, enhances efficiency, and adapts to a wide range of scientific research scenarios.
Instruction for use
1. ChIP-Grade Protein A/G Magnetic Agarose Beads can be used in experiments such as ChIP and RIP.
2. Approximately 50–80 µl of ChIP-Grade Protein A/G Magnetic Agarose Beads are used per reaction.
3. Magnetic separation: Place the tube on a magnetic stand for 10 seconds, then remove the supernatant.
Matters needing attention
1. This product should be maintained at pH 6–8. Avoid high-speed centrifugation and drying. Do not leave the beads in a magnetic field for extended periods, as this may cause bead aggregation.
2. Prior to use, the beads should be properly and thoroughly resuspended by inverting the tube several times to ensure homogeneous mixing. Mix gently, avoiding vigorous vortexing or shaking to prevent protein denaturation.
3. If using instruments such as a vacuum pump to aspirate the supernatant, carefully control the suction intensity to avoid aspirating aggregated beads due to excessive force.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | C1507450 | |
| 分析证书 | C1507450 |
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