环丙沙星 (Ciprofloxacin)属于一种高效广谱抗生素,CAS号为85721-33-1,分子量为331.35,其原理在于抑制细菌DNA解旋酶,阻止细菌复制,可以快速降低细菌繁殖,是杀菌型抗菌药。除喹诺酮类外,环丙沙星与其它任何抗生素有不同作用方式,对青霉素类、头孢菌素类、氨基糖苷类和四环素类耐药菌珠均显较高抗菌能力。临床上,对肠杆菌、绿脓杆菌、流感嗜血杆菌、淋球菌、链球菌、军团菌、金黄色葡萄球菌具有抗菌作用。盐酸环丙沙星是环丙沙星的盐酸盐形式,其cas号是86393-32-0。
盐酸环丙沙星溶液 (20 mg/ml)为无菌溶液,所用原料为环丙沙星盐酸盐而非环丙沙星,具广谱抗菌活性,杀菌效果好,几乎对所有细菌具有抗菌活性,可以用于体外药物杀灭实验。该试剂仅用于生物科研领域,不适用于临床诊断或其他用途。
操作步骤 (仅供参考):
1、根据实验具体要求操作。
2、一般工作浓度为50 μg/ml,即400倍稀释使用。
注意事项:
1、注意无菌操作,避免交叉污染。
2、尽量减少反复冻融的次数,以免效率下降。
3、试剂开封后请尽快使用,以防影响后续实验效果。
4、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Ciprofloxacin is a highly effective broad-spectrum antibiotic with a CAS number of 85721-33-1 and a molecular weight of 331.35. Its mechanism of action lies in inhibiting bacterial DNA gyrase, blocking bacterial replication, and rapidly reducing bacterial proliferation, thus classifying it as a bactericidal antimicrobial agent. With a mode of action distinct from all other antibiotics except quinolones, ciprofloxacin exhibits strong antibacterial activity against bacterial strains resistant to penicillins, cephalosporins, aminoglycosides, and tetracyclines. Clinically, it exerts antibacterial effects on Enterobacter, Pseudomonas aeruginosa, Haemophilus influenzae, Neisseria gonorrhoeae, Streptococcus, Legionella, and Staphylococcus aureus. Ciprofloxacin hydrochloride is the hydrochloride form of ciprofloxacin, with a CAS number of 86393-32-0.
Ciprofloxacin Hydrochloride Solution (20 mg/ml) is a sterile solution formulated with ciprofloxacin hydrochloride instead of ciprofloxacin base. It possesses broad-spectrum antibacterial activity and excellent bactericidal effect, exhibiting inhibitory activity against nearly all bacterial species. This solution can be used for in vitro drug killing assays. This reagent is for biological research use only and not intended for clinical diagnosis or any other applications.
Operating Procedures (For Reference Only)
1. Operate in accordance with the specific requirements of the experiment.
2. The general working concentration is 50 μg/ml, which can be achieved by a 1:400 dilution of the stock solution.
Precautions
1. Perform strict aseptic techniques to avoid cross-contamination.
2. Minimize the number of repeated freeze-thaw cycles to prevent reduced efficacy.
3. Use the reagent as soon as possible after opening to avoid adverse effects on subsequent experimental results.
4. For your safety and health, wear a lab coat and disposable gloves during operation.